Molecular tools that target RNA at specific sites allow recoding of RNA information and processing. SNAP-tagged deaminases guided by a chemically stabilized guide RNA can edit targeted adenosine to inosine in several endogenous transcripts simultaneously, with high efficiency (up to 90%), high potency, sufficient editing duration, and high precision. We used adenosine deaminases acting on RNA (ADARs) fused to SNAP-tag for the efficient and concurrent editing of two disease-relevant signaling transcripts, KRAS and STAT1. We also demonstrate improved performance compared with that of the recently described Cas13b–ADAR.

中文翻译：

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带有SNAP标签的ADAR可以高效，精确地编辑内源转录本。

将RNA靶向特定位点的分子工具可以重新编码RNA信息并进行加工。由化学稳定的引导RNA引导的带有SNAP标签的脱氨酶可以同时在多个内源性转录物中将目标腺苷编辑为肌苷，效率高（高达90％），高效，足够的编辑时间和高精度。我们使用作用于与SNAP-tag融合的RNA（ADARs）的腺苷脱氨基酶，有效并发地编辑了两种与疾病相关的信号转录本，即KRAS和STAT1。与最近描述的Cas13b–ADAR相比，我们还展示了更高的性能。