Abstract Here, we engineered spheroids by using ECM mimicking nano-fragments (NFs) with fibroblasts and investigated their effect on proliferation and cell/ECM interactions. NF incorporation resulted in formation of a stable spheroid, which improved proliferation and viability of cells by assisting oxygen transport confirmed by LOX-1 staining. In addition, hypoxic and apoptotic genes were significantly downregulated in spheroids with PD-NFs. Furthermore, ECM and cell junction proteins were highly expressed. Overall, our findings suggest that incorporation of NFs within spheroids for assembly with various cell types can be an alternative approach for 3D cell culture in many applications.

中文翻译：

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人真皮成纤维细胞与 ECM 模拟纳米片段的团聚及其对增殖和细胞/ECM 相互作用的影响

摘要 在这里，我们通过使用带有成纤维细胞的 ECM 模拟纳米片段 (NFs) 设计了球体，并研究了它们对增殖和细胞/ECM 相互作用的影响。NF 掺入导致形成稳定的球体，通过帮助 LOX-1 染色证实的氧运输来改善细胞的增殖和活力。此外，在具有 PD-NFs 的球体中，缺氧和凋亡基因显着下调。此外，ECM 和细胞连接蛋白高度表达。总的来说，我们的研究结果表明，在球体中加入 NFs 以与各种细胞类型组装可以成为许多应用中 3D 细胞培养的替代方法。