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Specific Two-Photon Imaging of Live Cellular and Deep-Tissue Lipid Droplets by Lipophilic AIEgens at Ultralow Concentration
Chemistry of Materials ( IF 8.6 ) Pub Date : 2018-07-02 00:00:00 , DOI: 10.1021/acs.chemmater.8b01943
Guangle Niu 1, 2 , Ruoyao Zhang 2, 3 , John P. C. Kwong 1, 2 , Jacky W. Y. Lam 1, 2 , Congping Chen 4 , Jianguo Wang 1, 2 , Yuncong Chen 1, 2 , Xing Feng 1, 2 , Ryan T. K. Kwok 1, 2 , Herman H.-Y. Sung 2 , Ian D. Williams 2 , Mark R. J. Elsegood 5 , Jianan Qu 4 , Chao Ma 6 , Kam Sing Wong 6 , Xiaoqiang Yu 3 , Ben Zhong Tang 1, 2, 7
Affiliation  

Lipid droplets are highly associated with obesity, diabetes, inflammatory disorders, and cancer. A reliable two-photon dye for specific lipid droplets imaging in live cells and live tissues at ultralow concentration has rarely been reported. In this work, four new aggregation-induced emission luminogens (AIEgens) based on the naphthalene core were designed and synthesized for specific two-photon lipid droplet staining. The new molecules, namely, NAP AIEgens, exhibit large Stokes shift (>110 nm), high solid-state fluorescence quantum yield (up to 30%), good two-photon absorption cross section (45–100 GM at 860 nm), high biocompatibility, and good photostability. They could specifically stain lipid droplets at ultralow concentration (50 nM) in a short time of 15 min. Such ultralow concentration is the lowest value for lipid droplets staining in live cells reported so far. In vitro and ex vivo two-photon imaging of lipid droplets in live cells and live mice liver tissues were successfully demonstrated. In addition, selective visualization of lipid droplets in live mice liver tissues could be achieved at a depth of about 70 μm. These excellent properties render them as promising candidates for investigating lipid droplet-associated physiological and pathological processes in live biological samples.

中文翻译:

亲脂性AIEgens在超低浓度下对活细胞和深层组织脂质小滴的特定双光子成像

脂质滴与肥胖,糖尿病,炎性疾病和癌症高度相关。很少有人报道过一种可靠的双光子染料,可以在活细胞和活组织中以超低浓度对特定的脂质小滴成像。在这项工作中,针对特定的双光子脂质液滴染色,设计并合成了四种基于萘核的新的聚集诱导发射发光剂(AIEgens)。新分子,即NAP AIEgens,表现出大的斯托克斯位移(> 110 nm),高的固态荧光量子产率(高达30%),良好的双光子吸收截面(在860 nm处为45-100 GM),高生物相容性和良好的光稳定性。他们可以在15分钟的短时间内以超低浓度(50 nM)对脂质滴进行特异性染色。成功地证明了活细胞和活小鼠肝组织中脂质滴的体外离体双光子成像。此外,可以在约70μm的深度选择性观察活小鼠肝脏组织中的脂质滴。这些优异的性能使其成为研究活生物样品中与脂质液滴相关的生理和病理过程的有前途的候选者。
更新日期:2018-07-02
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