Lipidomics is a rapidly developing field in modern biomedical research. While LC-MS systems are able to detect most of the known lipid classes in a biological matrix, there is no single technique able to extract all of them simultaneously. In comparison with two-phase extractions, one-phase extraction systems are of particular interest, since they decrease the complexity of the experimental procedure. By using an untargeted lipidomics approach, we explored the differences/similarities between the most commonly used two-phase extraction systems (Folch, Bligh and Dyer, and MTBE) and one of the more recently introduced one-phase extraction systems for lipid analysis based on the MMC solvent mixture (MeOH/MTBE/CHCl₃). The four extraction methods were evaluated and thoroughly compared against a pooled extract that qualitatively and quantitatively represents the average of the combined extractions. Our results show that the lipid profile obtained with the MMC system displayed the highest similarity to the pooled extract, indicating that it was most representative of the lipidome in the original sample. Furthermore, it showed better extraction efficiencies for moderate and highly apolar lipid species in comparison with the Folch, Bligh and Dyer, and MTBE extraction systems. Finally, the technical simplicity of the MMC procedure makes this solvent system highly suitable for automated, untargeted lipidomics analysis.

脂质组学是现代生物医学研究中一个快速发展的领域。尽管LC-MS系统能够检测生物基质中大多数已知的脂质类，但没有一种技术能够同时提取所有脂质类。与两相萃取相比，一相萃取系统特别受关注，因为它们降低了实验程序的复杂性。通过使用非靶向脂质组学方法，我们探索了最常用的两相萃取系统（Folch，Bligh和Dyer和MTBE）与最近推出的一种基于脂质分析的一相萃取系统之间的差异/相似性。 MMC溶剂混合物（MeOH / MTBE / CHCl ₃）。对这四种提取方法进行了评估，并与定性和定量表示合并提取物平均值的合并提取物进行了全面比较。我们的结果表明，使用MMC系统获得的脂质谱与合并提取物的相似性最高，表明它是原始样品中脂质组的最有代表性。此外，与Folch，Bligh和Dyer以及MTBE萃取系统相比，它对中度和高度非极性脂质种类的萃取效率更高。最后，MMC程序的技术简便性使得该溶剂系统非常适合自动化，非目标化脂质组学分析。