Abstract Previous studies have shown that heterocyst glycolipids (HGs) are unique markers for N2-fixing heterocystous cyanobacteria. In this study, the HGs of a marine pelagic Calothrix sp. CCY1611 isolated from the tropical western North Atlantic were analyzed by ultra-high pressure liquid chromatography–high resolution mass spectrometry and it was shown that this organism contains an unusual C28 triol HG with a methylated C6 sugar (methyl-HG28 triol) head group. Gas chromatography–mass spectrometry analysis of the sugar released from the novel HG by acid methanolysis revealed that the sugar is likely 6-O-methyl-β- d -glucopyranose. We propose that this methyl-HG28 triol is a potential biomarker for pelagic members of the genus Calothrix.

中文翻译：

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在 Calothrix 属的远洋 N 2 固定蓝藻中检测到一种新型杂囊糖脂

摘要 先前的研究表明，杂囊糖脂 (HG) 是固定 N2 的杂囊蓝细菌的独特标记物。在这项研究中，海洋中上层 Calothrix sp. 的 HG。通过超高压液相色谱-高分辨率质谱分析从热带西北大西洋分离出的 CCY1611，结果表明该生物体含有一种不寻常的 C28 三醇 HG，具有甲基化的 C6 糖（甲基-HG28 三醇）头基。通过酸甲醇分解从新型 HG 中释放的糖的气相色谱-质谱分析表明，该糖可能是 6-O-甲基-β-d-吡喃葡萄糖。我们建议这种甲基-HG28 三醇是 Calothrix 属中上层成员的潜在生物标志物。