Magnetic nanoparticles have been extensively explored for the development of platforms for drug delivery and imaging probes. In this work, we have used a modular capping strategy to produce magnetic gold-coated Fe₃O₄ (Fe₃O₄@Au) nanoparticles, which have been decorated with a copper (II) complex containing a thioether derivative of clip-phen (Fe₃O₄@[email protected]), where the complex [Cu(2CP-Bz-SMe)]²⁺ has affinity to bind DNA and proven nuclease activity (2CP-Bz-SMe=1,3-bis((1,10-phenanthrolin-2-yl)oxy)-N-(4-(methylthio)benzylidene)propan-2-imine). The functionalization of Fe₃O₄@Au with the copper complex occurs through the sulfur atom of the thioether moiety, as indicated by Raman scattering on surface. The magnetic measurements showed the nanomaterial Fe₃O₄@[email protected] is still magnetic although the gold shell and the functionalization with the copper complex have diminished the magnetization due to the dilution of the magnetic core. The nuclease assays performed with Fe₃O₄@[email protected] indicate that the nuclease activity of the nanomaterial toward the plasmid DNA involves an oxidative pathway in which H₂O₂ species is involved as intermediate in a Fenton-like reaction. Based on the electron paramagnetic resonance spectra (a^N = 15.07 G, a^H = 14.99 G), such nuclease activity is assigned, essentially, to the HO species indicating that the radical production property of [Cu(2CP-Bz-SMe)]²⁺ is successfully transferred to the core-shell gold-coated Fe₃O₄ magnetic nanoparticles. To the best of our knowledge, this is the first study reporting nuclease activity due to the reactive oxygen species generated by a copper complex immobilized on a gold-coated magnetic nanoparticle.

磁性纳米粒子已被广泛探索以开发用于药物递送和成像探针的平台。在这项工作中，我们使用了一种模块化的封盖策略来生产磁性镀金的Fe ₃ O ₄（Fe ₃ O ₄ @Au）纳米颗粒，该纳米颗粒已经用含有Clip-phen硫醚衍生物的铜（II）配合物修饰。 （Fe ₃ O ₄ @ [受电子邮件保护]），其中复合物[Cu（2CP-Bz-SMe）] ²⁺具有结合DNA的亲和力和已证明的核酸酶活性（2CP-Bz-SMe = 1,3-bis（（ 1,10-菲咯啉-2-基）氧基）-N-（4-（甲硫基亚苄基）丙烷-2-亚胺）。Fe ₃ O ₄的官能化如表面上的拉曼散射所示，具有铜配合物的@Au通过硫醚部分的硫原子发生。磁性测量表明，尽管金壳和铜配合物的官能化由于磁芯的稀释而降低了磁化强度，但是纳米材料Fe ₃ O ₄ @ [受电子邮件保护]仍然是磁性的。用Fe ₃ O ₄进行的核酸酶分析表明，纳米材料对质粒DNA的核酸酶活性涉及一种氧化途径，其中H ₂ O ₂物种参与了芬顿样反应。基于电子顺磁共振波谱（a ^N = 15.07 G，^H  = 14.99 G），这种核酸酶活性基本上分配给HO物种，表明[Cu（2CP-Bz-SMe）] ²⁺的自由基产生性质已成功转移到核-壳中镀金的Fe ₃ O ₄磁性纳米粒子。据我们所知，这是第一个报告核酸酶活性的研究，该酶由于固定在金涂层磁性纳米粒子上的铜络合物产生的活性氧而产生。