A majority of cellular diseases, independent of their origin, are characterized by a dramatic increase in Reactive Oxygen Species (ROS) in response to stress. In most cases, the uncontrolled detrimental ROS outburst is difficult to handle for the cellular machinery and eventually leads to cell mortality. In this study, we compare the antioxidant efficacy of quercetin and melatonin to find out a better alternative against lipopolysaccharide (LPS) induced tissue injury by oxidative stress in Funambulus pennanti. Transient exposure to LPS significantly increased ROS generation and lipid peroxidation levels in bone marrow mononuclear cells (MNCs) and spleen which was further corroborated by decreased activities of SOD, CAT and Gpx enzymes. It also downregulate the expression of cellular oxidative stress response proteins Nrf-2 and HO-1 in spleen and decreases the proliferation of bone marrow derived Granulocyte macrophage-colony forming unit cells (GM-CFU). Both melatonin and quercetin pre-treatments rescued these effects, however, our results indicated that the efficacy of melatonin to overcome oxidative stress was significantly better than quercetin. Our findings support the idea that melatonin is a better antioxidant and immunomodulator as compared to other alternatives and perhaps may be employed in the development of effective therapeutics against ROS dominated diseases.

大多数细胞疾病，不论其起源如何，其特征都是响应压力而使活性氧（ROS）急剧增加。在大多数情况下，细胞机械难以控制不受控制的有害ROS爆发，最终导致细胞死亡。在这项研究中，我们比较了槲皮素和褪黑素的抗氧化功效，以找到更好的替代方法来对抗脂多糖（LPS）引起的Funambulus pennanti氧化应激引起的组织损伤。短暂暴露于LPS会显着增加骨髓单核细胞（MNC）和脾脏中ROS的产生和脂质过氧化水平，这进一步被SOD，CAT和Gpx酶的活性降低所证实。它也下调脾脏中细胞氧化应激蛋白Nrf-2和HO-1的表达，并降低骨髓衍生的粒细胞巨噬细胞集落形成单位细胞（GM-CFU）的增殖。褪黑素和槲皮素预处理均能挽救这些效应，但是，我们的结果表明，褪黑素克服氧化应激的功效明显优于槲皮素。我们的发现支持这样的想法，即褪黑激素与其他替代品相比是更好的抗氧化剂和免疫调节剂，也许可以用于开发针对ROS为主的疾病的有效疗法。我们的结果表明褪黑素克服氧化应激的功效明显优于槲皮素。我们的发现支持这样的想法，即褪黑激素与其他替代品相比是更好的抗氧化剂和免疫调节剂，也许可以用于开发针对ROS为主的疾病的有效疗法。我们的结果表明褪黑素克服氧化应激的功效明显优于槲皮素。我们的发现支持这样的想法，即褪黑激素与其他替代品相比是更好的抗氧化剂和免疫调节剂，也许可以用于开发针对ROS为主的疾病的有效疗法。