Changes in lipid composition of cells or tissue are often linked to various diseases. Studies indicate alterations of bis(monoacylglycero)phosphate (BMP) species in diseases such as cancer. Therefore, an extended phospholipid profiling method based on hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution mass spectrometry (MS) and data-dependent MS/MS acquisition was developed to separate and unambiguously identify BMP species. Lipid species identification was based on retention time, accurate mass and specific MS/MS fragments. The developed method was applied in a proof of concept study to lipid extracts of a cell culture model of conditional oncogene overexpression in MCF-7/NeuT breast cancer cells. Comparison of control and oncogene-induced MCF-7/NeuT breast cancer cells showed changes in BMP species distribution. Thereby, a shift from long-chain to shorter-chain fatty acid composition in BMP species was detected.

细胞或组织脂质组成的变化通常与各种疾病有关。研究表明，在癌症等疾病中，双（单酰基甘油）磷酸酯（BMP）种类发生了变化。因此，开发了基于亲水相互作用液相色谱（HILIC）结合高分辨率质谱（MS）和数据依赖的MS / MS采集的扩展磷脂谱分析方法，以分离和明确鉴定BMP种类。脂质种类的鉴定是基于保留时间，准确的质量数和特定的MS / MS片段。所开发的方法在概念验证研究中应用于MCF-7 / NeuT乳腺癌细胞中条件致癌基因过度表达的细胞培养模型的脂质提取物。对照和癌基因诱导的MCF-7 / NeuT乳腺癌细胞的比较显示BMP物种分布发生了变化。