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Ferulic acid inhibits advanced glycation end products (AGEs) formation and mitigates the AGEs-induced inflammatory response in HUVEC cells
Journal of Functional Foods ( IF 5.6 ) Pub Date : 2018-06-29 , DOI: 10.1016/j.jff.2018.06.024
Jian-li Liu , Yong-lin He , Shuai Wang , Yin He , Wei-yu Wang , Qi-jiu Li , Xiang-yu Cao

Advanced glycation end products (AGEs) are complex and heterogeneous compounds, which play an important role in diabetic-related vascular complications, especially in atherosclerosis. In this study, the effects of ferulic acid (FA) on glucose-related protein glycation were investigated in vitro by various spectroscopic techniques and molecular docking methods. In order to study the protective effects of FA on AGEs-induced HUVEC cells damage, the mRNA expression of Caspase-1, NLRP3, CRP, ICAM-1, VCAM-1, IL-18 and IL-1β were detected. Moreover, NF-κB and p38 MAPK signaling pathways were analyzed. These results manifested that FA could effectively inhibit the AGEs formation, decrease the AGEs-induced mRNA expression of Caspase-1, NLRP3, CRP, ICAM-1, VCAM-1, IL-18 and IL-1β, and reduce intracellular ROS. FA could mitigate the AGEs-induced inflammatory response in HUVEC cells by suppressing the activation of NF-κB and p38 MAPK signaling pathways. These results suggested that FA might prevent and mitigate the development of atherosclerosis in diabetic patients.



中文翻译:

阿魏酸抑制HUVEC细胞中晚期糖基化终产物(AGEs)的形成并减轻AGEs诱导的炎症反应

晚期糖基化终产物(AGEs)是复杂的异质化合物,在糖尿病相关的血管并发症,尤其是动脉粥样硬化中起着重要的作用。在这项研究中,通过各种光谱技术和分子对接方法,体外研究了阿魏酸(FA)对葡萄糖相关蛋白糖基化的影响。为了研究FA对AGEs诱导的HUVEC细胞损伤的保护作用,检测了Caspase-1,NLRP3,CRP,ICAM-1,VCAM-1,IL-18和IL-1β的mRNA表达。此外,分析了NF-κB和p38 MAPK信号通路。这些结果表明FA可以有效抑制AGEs的形成,降低AGEs诱导的Caspase-1,NLRP3,CRP,ICAM-1,VCAM-1,IL-18和IL-1β的mRNA表达,并降低细胞内ROS。FA可以通过抑制NF-κB和p38 MAPK信号通路的激活来减轻AGEs诱导的HUVEC细胞的炎症反应。这些结果表明FA可能预防和减轻糖尿病患者的动脉粥样硬化的发展。

更新日期:2018-06-29
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