Kinetic optimization of continuous liquid-liquid extraction (CLLE) can shorten sample preparation times and reduce losses of labile or volatile analytes. Here, we coupled a downscaled CLLE apparatus with atmospheric pressure chemical ionization interface of triple quadrupole mass spectrometer. Real-time sampling was guided by an Arduino-based programmable logic controller. The recorded datasets were processed to compute the extraction rate constants for the target analytes. The extraction time in subsequent on-line experiments was set to 180 min as a compromise between the reduction of the analysis time and maximizing its yield. Interestingly, off-line analysis of the extract produced different results than on-line analysis pointing to the immanent degradation of the collected extract aliquots. Next, we implemented this hyphenated system in the analysis of red wine samples, which were stored during different periods of time after opening the bottle. The results reveal differences in the depletion of the volatile wine components during storage.

中文翻译：

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实时检测葡萄酒连续液液萃取动力学研究

连续液液萃取 (CLLE) 的动力学优化可以缩短样品制备时间并减少不稳定或挥发性分析物的损失。在这里，我们将缩小比例的 CLLE 装置与三重四极杆质谱仪的大气压化学电离接口相结合。实时采样由基于 Arduino 的可编程逻辑控制器引导。处理记录的数据集以计算目标分析物的提取速率常数。在随后的在线实验中，提取时间设置为 180 分钟，这是在减少分析时间和最大化其产量之间的折衷。有趣的是，提取物的离线分析产生了与在线分析不同的结果，表明所收集的提取物等分试样的内在降解。下一个，我们在分析红酒样品时实施了这种联用系统，这些样品在开瓶后的不同时间段内储存。结果揭示了储存过程中挥发性葡萄酒成分消耗的差异。