The analysis of protein interaction networks is one of the key challenges in the study of biology. It connects genotypes to phenotypes, and disruption often leads to diseases. Hence, many technologies have been developed to study protein‐protein interactions (PPIs) in a cellular context. The expansion of the PPI technology toolbox however complicates the selection of optimal approaches for diverse biological questions. This review gives an overview of the binary and co‐complex technologies, with the former evaluating the interaction of two co‐expressed genetically tagged proteins, and the latter only needing the expression of a single tagged protein or no tagged proteins at all. Mass spectrometry is crucial for some binary and all co‐complex technologies. After the detailed description of the different technologies, the review compares their unique specifications, advantages, disadvantages, and applicability, while highlighting opportunities for further advancements.

中文翻译：

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发现细胞蛋白相互作用：技术策略和机遇

蛋白质相互作用网络的分析是生物学研究中的关键挑战之一。它将基因型与表型联系起来，破坏通常会导致疾病。因此，已经开发了许多技术来研究细胞环境中的蛋白质-蛋白质相互作用（PPI）。但是，PPI技术工具箱的扩展使针对各种生物学问题的最佳方法的选择变得复杂。本文对二元技术和共复杂技术进行了概述，前者评估了两种共表达的遗传标记蛋白的相互作用，而后者仅需要表达单个标记蛋白或根本不需要表达蛋白。质谱对于某些二进制和所有复杂的技术至关重要。在详细描述了不同的技术之后，