Cell encapsulation using microgel and nanogel, as a strategy of cell surface engineering, can mimic the niches of cells and organoids. The established niche that seasons cells and tissues for the controllable development underlies the superiority of encapsulation on cells. Encapsulation by layer-by-layer nanogel coating is a bottom-up simulation of extracellular matrices via nano- or micropackaging of cells in a multiscale way. We report the nanogel encapsulation on individual neuronal cell for a basic study and application of permeability tuning to regulate cells’ apoptosis. Gelatin and hyaluronic acid (HA) are applied for encapsulating PC12 cells. The permeability of encapsulation on cells can be managed by adjusting different parameters such as material concentration, layer thickness and environmental pH. Eventually, permeability of tumor necrosis factor-α (TNF-α) is controlled by tuning encapsulating parameters for blocking the interaction with TNF-receptor 1, so that cell apoptosis is inhibited. In short, nanogel encapsulation exhibits controllable permeability to different molecules and exerts screen effect on TNF-α for protection. This technique holds great potential in basic biological research and translational research, for example, the protection of transplanted cells against apoptotic factors in target areas.

中文翻译：

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纳米胶囊对细胞的可操纵渗透性具有针对TNF-α诱导的细胞凋亡的保护作用。

使用微凝胶和纳米凝胶进行细胞包封，作为细胞表面工程学的一种策略，可以模仿细胞和类器官的壁ches。为细胞和组织的可控发育而调味的既定利基，奠定了细胞上包囊的优越性。通过逐层纳米凝胶涂层的封装是通过细胞的纳米或微包装以多尺度方式对细胞外基质进行的自下而上的模拟。我们报告纳米凝胶封装在单个神经元细胞上的基础研究和通透性调整以调节细胞凋亡的应用。明胶和透明质酸（HA）用于封装PC12细胞。可以通过调整不同的参数（例如材料浓度，层厚度和环境pH）来管理封装在细胞上的渗透性。最终，肿瘤坏死因子-α（TNF-α）的通透性通过调节用于阻断与TNF-受体1相互作用的包囊参数来控制，从而抑制细胞凋亡。简而言之，纳米凝胶包封对不同分子表现出可控的渗透性，并对TNF-α起到屏蔽作用，从而起到保护作用。该技术在基础生物学研究和转化研究中具有巨大潜力，例如，保护移植细胞免受靶区域中凋亡因子的影响。