A method based on matrix solid-phase dispersion (MSPD) associated to gas chromatography-flame photometric detection (GC-FPD), GC-electron capture detection (GC-ECD) and GC-mass spectrometry (GC–MS) for confirmation purposes, was developed for the determination of a representative group of twelve pesticides in honeybee with particular concern in the apicultural field (fipronil, thiamethoxam, acetamiprid, acrinathrin, metamidophos, dimetoathe, diazinon, chlorpyrifos, methidathion, profenophos, azinphos methyl and coumaphos). Factors influencing the extraction efficiency of MSPD were investigated and optimized through response surface method. The use of octadecylsilyl (C18) sorbent combined with a florisil clean-up and acetonitrile-methanol (99:1) elution was the optimal condition for the extraction of the selected pesticides. Under this condition the recovery of pesticides at the limit of quantification of the method (0.007 to 0.050 μg g⁻¹) ranged from 68 to 102% with RSDs for within-laboratory reproducibility ≤20%. The proposed method was applied to the analysis of honeybees collected in 68 field hives from areas of great apicultural and agricultural development in central Chile. In 65% of these samples eight different pesticides were detected. Pesticides most frequently found were chlorpyrifos (34% of the samples, <0.017–0.067 μg g⁻¹), acrinathrin (32% of the samples, <0.020–0.026 μg g⁻¹) and diazinon (10% of the samples at values <0.015 μg g⁻¹). The incidence of these pesticides in bees can be related to their high employ in central Chile, use to combat the varroosis in hives and hydrophobicity.

一种基于基质固相分散体（MSPD）的方法，用于气相色谱-火焰光度检测（GC-FPD），GC电子捕获检测（GC-ECD）和GC-质谱（GC-MS），用于确认目的，该试剂盒是为确定蜜蜂中代表性的十二种农药而开发的，尤其是在养蜂领域（氟虫腈，噻虫嗪，扑热息痛，草胺，间甲mid，敌百虫，二嗪农，毒死rif，甲硫磷，普罗非膦，甲基谷硫磷和库玛磷）。通过响应面法研究和优化了影响MSPD提取效率的因素。十八烷基甲硅烷基（C18）吸附剂与弗洛西尔净化剂和乙腈-甲醇（99：1）洗脱的组合使用是提取所选农药的最佳条件。^-1）范围从68％到102％，实验室内再现性的RSD≤20％。所提出的方法用于分析智利中部养蜂业和农业发达地区的68个田间蜂房中收集的蜜蜂。在这些样品的65％中，检测到8种不同的农药。最常见的农药是毒死rif（占样本的34％，<0.017–0.067μgg ^-1），草胺（占样本的32％，<0.020–0.026μgg ^-1）和二嗪农（在该值下占样本的10％） <0.015μgg ^-1）。这些农药在蜜蜂中的发生可能与其在智利中部的大量使用有关，可用于防治荨麻疹和疏水性。