Despite some recent developments on the portable on-site sensor of Aflatoxin B1 (AFB1), the complex and expensive preparation of recognition elements have still limited their wide applications. In this paper, using the fast, low-cost, and stable recognition of aptamer DNA-AFB1, a portable aptasensor was constructed for the on-site detection of AFB1 in food matrixes, with the readout of personal glucose meter (PGM) and DNA walking machine for signal probe separation. In such an assay protocol, the target could trigger the DNA walker to autonomously move on the electrode surface, propelled by unidirectional Pb²⁺-specific DNAzyme digestion, which could amplify the signal and separate the signal probe as well for further quantification by the PGM. Under optimized conditions, the increase of PGM signal was relative with the concentration of AFB1 ranging from 0.02 to 10 nM and the low limit of detection (LOD) was 10 pM (S/N = 3). With the features of portability, and cheapness, the presented user-friendly method could be extended to various other analytes for wide point-of-care applications.

中文翻译：

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基于个人血糖仪和DNA行走仪的便携式黄曲霉毒素B1在面包中的自适应传感器

尽管最近在黄曲霉毒素B1（AFB1）便携式现场传感器方面取得了一些进展，但是识别元件的复杂而昂贵的制备仍然限制了它们的广泛应用。在本文中，利用对适体DNA-AFB1的快速，低成本和稳定的识别，构建了一种便携式适体传感器，用于现场检测食品基质中的AFB1，并读出个人血糖仪（PGM）和DNA步行机，用于信号探头分离。在这种测定方案中，靶标可能触发DNA Walker在单向Pb ²⁺推动下在电极表面自主移动。特异性的DNA酶消化，可以放大信号并分离信号探针，以供PGM进一步定量。在最佳条件下，PGM信号的增加与AFB1的浓度在0.02至10 nM之间有关，而检测下限（LOD）为10 pM（S / N = 3）。由于具有便携性和价格便宜的特点，所提供的用户友好方法可以扩展到广泛的即时医疗应用中的其他各种分析物。