Ferroptosis is an iron-mediated, caspase-independent pathway of cell death that is accompanied with the accumulations of reactive oxygen species (ROS) and oxygenases, as well as being involved in many other pathophysiological procedures. However, specific and rapid monitoring of ferroptosis in living cells or tissues has not been achieved so far. Herein, a quinoxalinone-based fluorescent probe (termed as Quinos-4, or QS-4) with a reactive aromatic thioether moiety was designed for ferroptosis identification. Upon exposing it to high levels of ROS and hemeoxygenase-1 (HO-1), which are considered as the biochemical characteristics of ferroptosis, QS-4 could be oxidized into a sulfoxide derivative (QSO-4) and its original aggregation-induced enhanced red fluorescence emission could be converted to green fluorescence emission sharply. On the basis of this unique reaction-induced color conversion, this molecular probe can be employed for identifying the occurrence of ferroptosis both in vitro and in vivo.

中文翻译：

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基于反应的颜色可转换荧光探针用于鉴定铁锈病

Ferroptosis是一种铁介导的，不依赖caspase的细胞死亡途径，伴随着活性氧（ROS）和加氧酶的积累，并参与许多其他病理生理过程。但是，到目前为止，尚未实现对活细胞或组织中的铁锈病的专门且快速的监测。在此，设计具有反应性芳族硫醚部分的基于喹喔啉酮的荧光探针（称为Quinos-4或QS-4）用于铁锈病的鉴定。将其暴露于高水平的ROS和血氧合酶-1（HO-1）（被认为是肥大病的生化特征）后，QS-4可以被氧化成亚砜衍生物（QSO-4），并且其最初的聚集诱导作用得以增强。红色荧光发射可以急剧地转换为绿色荧光发射。