Tyrosinase (TYR), a key enzyme in biosynthesis of melanin, usually functions as a biomarker of severe skin diseases such as vitiligo and melanoma cancer. Accurate detection of TYR activity in vivo is urgent but still challenging. Inspired by the advantages of bioluminescence in vivo strategy in imaging and the specific hydroxylation of 3-hydroxybenzyloxy group by TYR, a bioluminogenic probe, TYR–LH₂, was designed and synthesized through caging D-luciferin with 3-hydroxybenzyl. The probe exhibits high selectivity and sensitivity toward TYR with a detection limit of 0.11 U/mL in a small detection volume of 100 μL. Bioluminescence imaging results show that TYR–LH₂ is fully competent for monitoring the dynamic changes of TYR in living cells and model animals and possesses the capability of discriminating melanocytes from other cell lines, thus offering a promising approach for investigation and diagnosis of melanoma cancer and other TYR-related diseases in vivo.

中文翻译：

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3-羟基苄基笼中的D-萤光素对体内酪氨酸酶的特异性成像

酪氨酸酶（TYR）是黑色素生物合成中的关键酶，通常可作为严重皮肤疾病（如白癜风和黑色素瘤癌症）的生物标志物。体内TYR活性的准确检测是紧迫的，但仍具有挑战性。受到生物发光体内成像策略的优势以及TYR对3-羟基苄氧基的特异性羟基化作用的启发，设计并合成了一种生物发光探针TYR–LH ₂，方法是将D-荧光素与3-羟基苄基化合物一起笼罩。该探针在100μL的小检测体积中对TYR表现出高选择性和灵敏度，检测限为0.11 U / mL。生物发光成像结果表明，TYR–LH ₂ 完全有能力监测活细胞和模型动物中TYR的动态变化，并具有区分黑素细胞与其他细胞系的能力，从而为体内研究和诊断黑素瘤癌症及其他TYR相关疾病提供了有希望的方法。