This paper describes a simple approach to develop a multifunctional chlorin e6-hyaluronic-Fe₃O₄ (Ce6-HA-Fe₃O₄) theranostic agent. Oleylamine-coated Fe₃O₄ nanoparticles were prepared through a thermal decomposition route and subsequently transformed into an aqueous phase using hyaluronic acid (HA) via an ultrasonic-assisted emulsion approach. The functional terminal carboxyl groups of the HA outer layer not only promoted the water solubility of Fe₃O₄ NPs and their targeting ability towards the CD44 overexpressing cancer cells but also allowed straightforward coupling with Ce6. The tumor targeting ability and cellular uptake of the theranostic agent have been demonstrated by magnetic resonance imaging (MRI), confocal microscopy, and flow cytometry (FCM) studies. FCM analysis allowed us to quantify the cellular uptake behavior in response to increased Ce6-HA-Fe₃O₄ concentration. While no significant toxicity was observed, cell fluorescence signals increased strongly in correlation with the concentration. Analysis on the photodynamic therapeutic (PDT) efficiency tested on B16F1 cells revealed 76.7% cell death under laser irradiation for 10 min (671 nm, 1 W), which is attributed to the successful co-delivery of active Ce6 using HA-Fe₃O₄ nanocarriers. Taken together, these results indicate that the multifunctional platform retained and combined the original magnetic, fluorescence, and PDT performance of the components in a single remotely triggered nanotheranostic agent. We anticipate that the theranostic agent Ce6-HA-Fe₃O₄ can act as a promising targeted dual modal probe and a PDT agent to enhance diagnosis and treatment of cancer.

中文翻译：

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荧光引导的磁性纳米载体，用于增强靶向肿瘤的光动力疗法†

本文介绍了一种开发多功能二氢卟酚e6-透明质酸-Fe ₃ O ₄（Ce6-HA-Fe ₃ O ₄）治疗剂的简单方法。通过热分解途径制备了油酸胺包覆的Fe ₃ O ₄纳米颗粒，随后使用透明质酸（HA）通过超声辅助乳液法将其转化为水相。HA外层的功能性末端羧基不仅促进了Fe ₃ O _4的水溶性NPs及其对过表达CD44的癌细胞的靶向能力，但也可以与Ce6直接偶联。已通过磁共振成像（MRI），共聚焦显微镜和流式细胞术（FCM）研究证明了治疗药物的肿瘤靶向能力和细胞吸收能力。FCM分析使我们能够量化响应增加的Ce6-HA-Fe ₃ O ₄浓度的细胞摄取行为。虽然未观察到明显的毒性，但细胞荧光信号随浓度的增加而强烈增加。对B16F1细胞测试的光动力治疗（PDT）效率的分析表明，在激光照射10分钟（671 nm，1 W）下，有76.7％的细胞死亡，这归因于使用HA-Fe成功地共同递送了活性Ce6₃ O ₄纳米载体。综上所述，这些结果表明多功能平台在单个远程触发的纳米热试剂中保留并结合了组件的原始磁性，荧光和PDT性能。我们预期治疗治疗药物Ce6-HA-Fe ₃ O ₄可以作为有前途的靶向双峰探针和PDT药物来增强癌症的诊断和治疗。