Nicotine is a major toxic alkaloid in wastes generated from tobacco production and cigarette manufacturing. In the present work, a nicotine-degrading bacterial strain was isolated from tobacco powdery waste. The isolate was identified as Pseudomonas sp. S-1 based on morphology, physiology, and 16S rRNA gene sequence. Suitable conditions of isolate S-1 for nicotine degradation were pH 7.0 and 30 °C. Catabolic intermediates of nicotine were isolated with preparative-HPLC and characterized with LC-HRMS and NMR. The catabolic pathways of nicotine were involved in dehydrogenation, oxidation, hydrolysis, and hydroxylation. Interestingly, nicotine catabolism in strain S-1 undergoes a new pyrrolidine pathway that differs from the other three catabolic pathways in bacterial species. This work sheds light on catabolic diversity of nicotine and heteroaromatics.

中文翻译：

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通过假单胞菌属物种中的新型吡咯烷途径对尼古丁的代谢进行表征。1号

尼古丁是烟草生产和卷烟生产产生的废物中的主要有毒生物碱。在目前的工作中，从烟草粉状废物中分离了尼古丁降解菌。该分离物被鉴定为假单胞菌sp。S-1基于形态学，生理学和16S rRNA基因序列。用于烟碱降解的分离物S-1的合适条件是pH 7.0和30°C。尼古丁的分解代谢中间体通过制备型HPLC分离，并通过LC-HRMS和NMR表征。尼古丁的分解代谢途径涉及脱氢，氧化，水解和羟基化。有趣的是，菌株S-1中的尼古丁分解代谢经历了一条新的吡咯烷途径，该途径不同于细菌物种中的其他三种分解代谢途径。这项工作揭示了尼古丁和杂芳族化合物的分解代谢多样性。