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Debranching of pea starch using pullulanase and ultrasonication synergistically to enhance slowly digestible and resistant starch
Food Chemistry ( IF 8.8 ) Pub Date : 2018-06-23 , DOI: 10.1016/j.foodchem.2018.06.115
Zhan-Hui Lu , Nicholas Belanger , Elizabeth Donner , Qiang Liu

Pullulanase (P) and ultrasonication (U) were simultaneously applied to debranch pea starch to enhance slowly digestible starch (SDS) and resistant starch (RS) fractions in the debranched pea starch (DPS). A synergistic debranching effect was found under conditions of pullulanase (40 npun/g) and ultrasonication (100% amplitude in pulse mode, 1 min on followed by 9 min off), which produced 73.5% linear glucans, 18% SDS and 26% RS in the resulting DPS-PU after 6 h of debranching. Even when autoclaving the DPS-PU at 118 °C for 30 min, following cooldown, 11% SDS and 25% RS were retained in the DPS-PU, compared with 0% SDS and 12% RS in autoclaved native pea starch. The SDS fraction in autoclaved DPS-PU further increased to 16% while the RS content remained constant during 14 days of cold storage. In summary, DPS-PU is high in linear glucans, low in starch digestibility and has a thermally stable RS fraction.



中文翻译:

使用支链淀粉酶和超声处理对豌豆淀粉进行脱支,以协同增强缓慢消化和抗性淀粉

同时应用支链淀粉酶(P)和超声处理(U)分离豌豆淀粉,以增强分离的豌豆淀粉(DPS)中的慢消化淀粉(SDS)和抗性淀粉(RS)组分。在支链淀粉酶(40 npun / g)和超声处理(脉冲模式下振幅为100%,先打开1分钟,再关闭9分钟)的条件下,发现了协同的脱支作用,产生了73.5%的线性葡聚糖,18%的SDS和26%的RS。脱支6小时后,所得的DPS-PU中的盐。即使在DPS-PU于118°C高压灭菌30分钟后,冷却后,DPS-PU中仍保留11%SDS和25%RS,而高压灭菌的天然豌豆淀粉中则为0%SDS和12%RS。高压灭菌的DPS-PU中的SDS分数进一步增加到16%,而在冷藏的14天中RS含量保持恒定。总而言之,DPS-PU的线性葡聚糖含量很高,

更新日期:2018-06-23
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