Indigenous strains of lactic acid bacteria previously isolated from raw donkey milk samples were used to ferment donkey milk. Each sample was subjected to an in vitro simulated gastrointestinal digestion (SGID) and analysis of the digesta by HPLC-LTQ-Orbitrap XL was performed in order to obtain a comprehensive peptide profile of fermented donkey milk. Functional properties such as ACE-inhibitory, antimicrobial and antioxidant activities of the resulting fermented donkey milks, as well as characterization of the bioactive peptides produced by an in vitro SGID, were assayed. All bioactivities were found to be high in fermented milk and a further significant increase was observed after SGID. The Enterococcus faecium DM33 fermented milk exhibited the strongest antioxidant and the highest antimicrobial activities. The highest ACE-inhibitory activity was observed in milk fermented with Lactobacillus casei DM214. These findings will contribute to the development of a new functional dairy drink with anti-hypertensive, antimicrobial and/or antioxidant activities.

先前从生驴乳样品中分离出的乳酸菌的本地菌株用于发酵驴乳。对每个样品进行体外模拟胃肠消化（SGID），并通过HPLC-LTQ-Orbitrap XL进行消化物分析，以获得发酵驴乳的全面肽谱。分析了所获得的发酵驴乳的功能特性，例如ACE抑制，抗微生物和抗氧化活性，以及​​通过体外SGID产生的生物活性肽的表征。发现发酵乳中所有生物活性都很高，SGID后观察到进一步的显着增加。在屎肠球菌DM33发酵乳展现出最强的抗氧化剂和最高的抗菌活性。在干酪乳杆菌DM214发酵的牛奶中观察到最高的ACE抑制活性。这些发现将有助于开发具有抗高血压，抗微生物和/或抗氧化活性的新型功能性乳饮料。