In this study, we investigated whether ZnO coating on Ag nanoparticles (NPs) tunes electron flux and hole figuration at the metal–semiconductor interface under UV radiation. This effect triggers the photoactivity and generation of reactive oxygen species from [email protected] NPs, which results in enhanced cytotoxic effects and apoptotic cell death in human breast cancer cells (MDA-MB231). In this context, upregulation of apoptotic cascade proteins (i.e., Bax/Bcl2 association, p53, cytochrome c, and caspase-3) along with activation of oxidative stress proteins suggested the occurrence of apoptosis by [email protected] NPs in cancer cells through the mitochondrial pathway. Also, preincubation of breast cancer cells with [email protected] NPs in dark conditions muted NP-related toxic effects and consequent apoptotic fate, highlighting biocompatible properties of unexcited [email protected] NPs. Furthermore, the diagnostic efficacy of [email protected] NPs as computed tomography (CT)/optical nanoprobes was investigated. Results confirmed the efficacy of the photoactivated system in obtaining desirable outcomes from CT/optical imaging, which represents novel theranostic NPs for simultaneous imaging and treatment of cancer.

中文翻译：

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[电子邮件保护]核壳纳米粒子的细胞内ROS诱导：乳腺癌治疗中永久性光学活性孔的前沿。

在这项研究中，我们研究了在银纳米颗粒（NPs）上的ZnO涂层是否能在紫外线辐射下调节金属-半导体界面上的电子通量和空穴形成。此效应触发了[受电子邮件保护的] NP的光活性和活性氧的生成，从而导致人乳腺癌细胞（MDA-MB231）的细胞毒性作用增强，并导致凋亡性细胞死亡。在这种情况下，凋亡级联蛋白（即Bax / Bcl2缔合，p53，细胞色素c和caspase-3）以及氧化应激蛋白的激活提示癌细胞通过线粒体途径通过[电子邮件保护的] NPs凋亡。同样，在黑暗条件下用[电子邮件保护的] NPs对乳腺癌细胞进行预温育会减弱与NP相关的毒性作用，并随之导致凋亡的命运，突出了未受[电子邮件保护的] NPs的生物相容性。此外，研究了[电子邮件保护] NPs作为计算机断层扫描（CT）/光学纳米探针的诊断功效。结果证实了光活化系统在从CT /光学成像中获得理想结果的功效，这代表了用于同时成像和治疗癌症的新型治疗治疗性NP。