We demonstrate a hybrid microfluidic system that combines fluidic trapping and acoustic switching to organize an array of single cells at high density. The fluidic trapping step is achieved by balancing the hydrodynamic resistances of three parallel channel segments forming a microfluidic trifurcation, the purpose of which was to capture single cells in a high-density array. Next, the cells were transferred into adjacent larger compartments by generating an array of streaming micro-vortices to move the cells to the desired streamlines in a massively parallel format. This approach can compartmentalize single cells with efficiencies of ≈67% in compartments that have diameters on the order of ∼100 um, which is an appropriate size for single cell proliferation studies and other single cell biochemical measurements.

中文翻译：

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用于组织高密度单细胞阵列的声流阱和传输方法†

我们展示了一种混合微流体系统，该系统结合了流体捕获和声学开关，以高密度组织单细胞阵列。流体捕获步骤是通过平衡形成微流体三叉的三个平行通道段的流体动力学阻力来实现的，其目的是捕获高密度阵列中的单个细胞。接下来，通过产生一系列流动微涡流，将细胞转移到相邻的较大隔室中，以大规模并行格式将细胞移动到所需的流线。这种方法可以以约 67% 的效率对直径约 100 um 的单细胞进行区室划分，这是单细胞增殖研究和其他单细胞生化测量的合适尺寸。