We present electrochemical evidence that magnetic interactions between the paramagnetic heme centers of fructose dehydrogenase subunit II and superparamagnetic iron oxide nanoparticles enable a suitable orientation of the enzyme molecule and enhance the rate of direct electron transfer. This enhancement of the rate of DET resulting from the appropriate orientation was examined with differential pulse voltammetry. The closer approach of the heme sites to the electrode results in a decreased fructose oxidation overpotential, a smaller Michaelis–Menten constant and an overall improvement in electrode performance in the presence of an external magnetic field.

中文翻译：

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磁场诱导的果糖脱氢酶在氧化铁纳米颗粒上的取向，以增强直接电子转移

我们目前的电化学证据表明，果糖脱氢酶亚基II和超顺磁性氧化铁纳米粒子的顺磁性血红素中心之间的磁性相互作用使酶分子具有合适的取向，并提高了直接电子转移的速率。通过差分脉冲伏安法检查了由适当的取向导致的DET速率的这种增强。血红素位点更靠近电极的方式导致果糖氧化超电势降低，Michaelis-Menten常数更小，并且在存在外部磁场的情况下整体改善了电极性能。