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Parapyruvate, an Impurity in Pyruvate Supplements, Induces Senescence in Human Fibroblastic Hs68 Cells via Inhibition of the α-Ketoglutarate Dehydrogenase Complex
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2018-06-22 00:00:00 , DOI: 10.1021/acs.jafc.8b01138 Shih-Chung Chang,Inn Lee,Hua Ting,Yuan-Jhe Chang,Nae-Cherng Yang
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2018-06-22 00:00:00 , DOI: 10.1021/acs.jafc.8b01138 Shih-Chung Chang,Inn Lee,Hua Ting,Yuan-Jhe Chang,Nae-Cherng Yang
Commercial dietary supplements of calcium pyruvate claim to be beneficial for losing weight, increasing muscle endurance, and regulating metabolism. Most industrial preparations have some impurities, including parapyruvate. Parapyruvate is an inhibitor of the α-ketoglutarate dehydrogenase complex (KGDHC). However, the effect and mechanism of parapyruvate on cell senescence and the content of parapyruvate in the dietary supplements of calcium pyruvate are unknown. In this study, we prepared pure parapyruvate with a purity of 99.8 ± 0.1% and investigated its ability to inhibit KGDHC activity and affect fibroblast senescence. Parapyruvate dose-dependently decreased KGDHC activity, with an IC50 of 4.13 mM and induced Hs68 cell senescence. Calcium ions, a KGDHC activator, antagonized the senescent effects of parapyruvate. The parapyruvate content was 1.4 ± 0.1% to 10.6 ± 0.2% in five brands of calcium pyruvate supplements. In this study, we showed that parapyruvate strongly induces Hs68 cell senescence by inhibiting KGDHC activity. Because of its KGDHC inhibition activity, the parapyruvate content should be an important issue for the food safety of calcium pyruvate supplements.
中文翻译:
副丙酮酸盐,丙酮酸补充剂中的杂质,通过抑制α-酮戊二酸脱氢酶复合物诱导人成纤维细胞Hs68细胞衰老。
丙酮酸钙的商业饮食补品声称对减肥,增加肌肉耐力和调节新陈代谢是有益的。大多数工业制剂都含有一些杂质,包括副丙酮酸盐。副丙酮酸是α-酮戊二酸脱氢酶复合物(KGDHC)的抑制剂。然而,丙酮酸丙酮酸对细胞衰老的作用和机理以及丙酮酸钙膳食补充剂中的丙酮酸含量是未知的。在这项研究中,我们制备了纯度为99.8±0.1%的纯副丙酮酸盐,并研究了其抑制KGDHC活性和影响成纤维细胞衰老的能力。副丙酮酸盐剂量依赖性降低KGDHC活性,IC 504.13 mM的诱导和诱导Hs68细胞衰老。钙离子是一种KGDHC活化剂,可拮抗副丙酮酸盐的衰老作用。在五个品牌的丙酮酸钙补充剂中副丙酮酸盐含量为1.4±0.1%至10.6±0.2%。在这项研究中,我们表明副丙酮酸通过抑制KGDHC活性强烈诱导Hs68细胞衰老。由于其具有对KGDHC的抑制活性,副丙酮酸盐的含量对于丙酮酸钙补充剂的食品安全性应该是一个重要的问题。
更新日期:2018-06-22
中文翻译:
副丙酮酸盐,丙酮酸补充剂中的杂质,通过抑制α-酮戊二酸脱氢酶复合物诱导人成纤维细胞Hs68细胞衰老。
丙酮酸钙的商业饮食补品声称对减肥,增加肌肉耐力和调节新陈代谢是有益的。大多数工业制剂都含有一些杂质,包括副丙酮酸盐。副丙酮酸是α-酮戊二酸脱氢酶复合物(KGDHC)的抑制剂。然而,丙酮酸丙酮酸对细胞衰老的作用和机理以及丙酮酸钙膳食补充剂中的丙酮酸含量是未知的。在这项研究中,我们制备了纯度为99.8±0.1%的纯副丙酮酸盐,并研究了其抑制KGDHC活性和影响成纤维细胞衰老的能力。副丙酮酸盐剂量依赖性降低KGDHC活性,IC 504.13 mM的诱导和诱导Hs68细胞衰老。钙离子是一种KGDHC活化剂,可拮抗副丙酮酸盐的衰老作用。在五个品牌的丙酮酸钙补充剂中副丙酮酸盐含量为1.4±0.1%至10.6±0.2%。在这项研究中,我们表明副丙酮酸通过抑制KGDHC活性强烈诱导Hs68细胞衰老。由于其具有对KGDHC的抑制活性,副丙酮酸盐的含量对于丙酮酸钙补充剂的食品安全性应该是一个重要的问题。