Objectives

To overcome shortcomings of hydraulic calcium-silicate cements (hCSCs), an experimental tricalcium silicate (TCS) cement, named ‘TCS 50’, was developed. In vitro research showed that TCS 50 played no negative effect on the viability and proliferation of human dental pulp cells, and it induced cell odontogenic differentiation. The objective was to evaluate the pulpal repair potential of TCS 50 applied onto exposed minipig pulps.

Methods

Twenty permanent teeth from three minipigs were mechanically exposed and capped using TCS 50; half of the teeth were scheduled for 7-day and the other half for 70-day examination (n = 10). Commercial hCSCs ProRoot MTA and TheraCal LC were tested as references (n = 8). Tooth discoloration was examined visually. After animal sacrifice, the teeth were scanned using micro-computed tomography; inflammatory response at day 7 and day 70, mineralized tissue formation at day 70 were assessed histologically.

Results

Up to 70 days, TCS 50 induced no discoloration, ProRoot MTA generated gray/black discoloration in all teeth. For TCS 50, 40.0% pulps exhibited a mild/moderate inflammation at day 7. No inflammation was detected and complete reparative dentin with tubular structures was formed in all pulps after 70 days. ProRoot MTA induced a similar response, TheraCal LC generated a less favorable response in terms of initial inflammation and reparative dentin formation; however, these differences were not significant (Chi-square test of independence: p > 0.05).

Significance

TCS 50 induced reparative dentinogenesis in minipig pulps. It can be considered as a promising pulp-capping agent, also for aesthetic areas.

中文翻译：

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实验性硅酸三钙水泥诱导修复性牙本质生成

目标

为了克服水硬性硅酸钙水泥（hCSCs）的缺点，开发了一种名为“ TCS 50”的实验性硅酸三钙（TCS）水泥。体外研究表明，TCS 50对人牙髓细胞的活力和增殖没有负面影响，并诱导细胞牙源性分化。目的是评估应用于暴露的小型猪牙髓的TCS 50的牙髓修复潜力。

方法

机械暴露三只迷你猪的二十颗恒牙，并用TCS 50盖好；一半的牙齿计划进行7天的检查，另一半计划进行70天的检查（n = 10）。测试了商用hCSC ProRoot MTA和TheraCal LC作为参考（n​​ = 8）。肉眼检查牙齿变色。处死动物后，使用微型计算机断层扫描技术扫描牙齿；在组织学上评估第7天和第70天的炎症反应，第70天的矿化组织形成。

结果

长达70天，TCS 50不会引起变色，ProRoot MTA在所有牙齿上都产生了灰色/黑色变色。对于TCS 50，在第7天有40.0％的牙髓表现出轻度/中度发炎，在70天后未检测到炎症，并且在所有牙髓中形成了具有管状结构的完整修复性牙本质。ProRoot MTA诱导了类似的反应，就初始炎症和修复性牙本质的形成而言，TheraCal LC产生的反应较差。但是，这些差异并不显着（独立性的卡方检验：p> 0.05）。

意义

TCS 50在小型猪牙髓中诱导修复性牙本质生成。对于美学领域，它也可以被认为是有前途的纸浆封盖剂。