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Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins
ACS Synthetic Biology ( IF 4.7 ) Pub Date : 2018-06-21 00:00:00 , DOI: 10.1021/acssynbio.8b00250
Elizaveta Chervyachkova 1 , Seraphine V. Wegner 1
Affiliation  

Toward the bottom-up assembly of synthetic cells from molecular building blocks, it is an ongoing challenge to assemble micrometer sized compartments that host different processes into precise multicompartmental assemblies, also called prototissues. The difficulty lies in controlling interactions between different compartments dynamically both in space and time, as these interactions determine how they organize with respect to each other and how they work together. In this study, we have been able to control the self-assembly and social self-sorting of four different types of colloids, which we use as a model for synthetic cells, into two separate families with visible light. For this purpose we used two photoswitchable protein pairs (iLID/Nano and nHagHigh/pMagHigh) that both reversibly heterodimerize upon blue light exposure and dissociate from each other in the dark. These photoswitchable proteins provide noninvasive, dynamic, and reversible remote control under biocompatible conditions over the self-assembly process with unprecedented spatial and temporal precision. In addition, each protein pair brings together specifically two different types of colloids. The orthogonality of the two protein pairs enables social self-sorting of a four component mixture into two distinct families of colloidal aggregates with controlled arrangements. These results will ultimately pave the way for the bottom-up assembly of multicompartment synthetic prototissues of a higher complexity, enabling us to control precisely and dynamically the organization of different compartments in space and time.

中文翻译:

具有蓝光可转换蛋白的胶体细胞模拟物的可逆社会自我分选

对于由分子构件自下而上地组装合成细胞而言,将微米级大小的容纳不同过程的小室组装成精确的多室组装(也称为原型组织)是一项持续的挑战。困难在于在空间和时间上动态地控制不同隔间之间的交互,因为这些交互决定了它们如何相对于彼此组织以及如何协同工作。在这项研究中,我们已经能够控制四种不同类型的胶体的自组装和社会自我分类,我们将它们用作合成细胞的模型,分为两个独立的可见光族。为此,我们使用了两个可光转换的蛋白质对(iLID / Nano和nHagHigh / pMagHigh),它们在蓝光照射下可逆地异源二聚体,并且在黑暗中彼此分离。这些光可开关蛋白在生物相容性条件下,以自组装过程提供了无创,动态和可逆的远程控制,具有空前的时空精度。另外,每个蛋白质对都将两种不同类型的胶体专门聚集在一起。两种蛋白质对的正交性可将四种成分的混合物进行社会自分类,形成具有可控排列的两个不同的胶体聚集体家族。这些结果将最终为复杂性更高的多隔室合成原生组织的自下而上的组装铺平道路,
更新日期:2018-06-21
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