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Analysis of terephthalate metabolites in human urine by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
Journal of Chromatography B ( IF 3 ) Pub Date : 2018-06-20 , DOI: 10.1016/j.jchromb.2018.06.044
Shedrack R. Nayebare , Rajendiran Karthikraj , Kurunthachalam Kannan

Owing to their toxicity, phthalate plasticizers are currently being replaced with terephthalates in many consumer products. Nevertheless, data on human exposure to and toxicity of terephthalates are still scarce. In this study, we developed a robust analytical method for the measurement of six terephthalate metabolites (TPhMs) in human urine through their successful separation from phthalate metabolites (PhMs). Target analytes were identified, using commercially available standards, and quantified with isotopically labeled internal standards (IS). The limits of quantification (LOQ) of TPhMs were in the range of 0.12 to 0.4 ng/mL, with the exception of 2.8 ng/mL for terephthalic acid (TPA) and 3.75 ng/mL for mono-(2-ethylhexyl) terephthalate (mEHTP), which were found in procedural blanks at notable levels. The method developed in this study showed excellent accuracy (recoveries: 86–117%) and precision (RSD: 0.6–12.2%) for TPhMs. The method was successfully applied for the analysis of 30 human urine samples collected from individuals with no known history of occupational exposure. The detection frequencies (df %) of TPhMs in urine ranged between 26.6 and 100%. This is one of the first studies that report a method for the analysis of emerging class of environmental chemicals in human specimens.



中文翻译:

高效液相色谱-串联质谱法(HPLC-MS / MS)分析人尿中的对苯二甲酸酯代谢物

由于其毒性,目前在许多消费产品中,邻苯二甲酸酯增塑剂已被对苯二甲酸酯替代。然而,关于人类对苯二甲酸酯的暴露和毒性的数据仍然很少。在这项研究中,我们开发了一种可靠的分析方法,可通过将尿中六种对苯二甲酸酯代谢物(Phhs)与邻苯二甲酸酯代谢物(PhMs)成功分离来进行测量。使用市售标准品鉴定目标分析物,并用同位素标记的内标物(IS)进行定量。TPhM的定量限(LOQ)在0.12至0.4 ng / mL的范围内,对苯二甲酸(TPA)的定量限为2.8 ng / mL,对苯二甲酸单-(2-乙基己基)酯的定量限为3.75 ng / mL( mEHTP),在程序空白中以显着水平被发现。在这项研究中开发的方法对TPhMs显示出极好的准确性(回收率:86–117%)和精确度(RSD:0.6–12.2%)。该方法已成功地用于分析30份从没有职业接触史的个人收集的人类尿液样品。检测频率(尿中TPhM的df%)介于26.6和100%之间。这是第一批报告了一种分析人类标本中新兴环境化学物质的方法的研究之一。

更新日期:2018-06-20
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