PLOS ONE ( IF 3.7 ) Pub Date : 2018-06-21 , DOI: 10.1371/journal.pone.0199495 Lukrecija Butorac , Katarina Hančević , Katarina Lukšić , Željko Škvorc , Mario Leko , Erika Maul , Goran Zdunić
The Eastern Adriatic region, encompassing Croatia and Bosnia and Herzegovina, is considered an important area of natural populations of wild grapevines (Vitis vinifera ssp. sylvestris). The wild grapevine arises in the Eastern Adriatic region in a contact zone of the EU-Mediterranean and the sub-Mediterranean characterized by typical karst relief. This study focuses on the chloroplast DNA (cpDNA) analysis of wild grapevines and the biodiversity of accompanying woody species to better understand the genetic variation of the sylvestris populations of the Eastern Adriatic region and to investigate how this variation fits within today’s wild grapevine distribution in the European continent. The allelic variation at nine cpDNA microsatellite loci of wild individuals was used to characterize haplotype diversity in 53 individuals from four population sites. All individuals were grouped into two chlorotypes: A and D, D being the rare haplotype among wild populations on the European continent. In total, 52 woody plant species were identified. However, the studied vegetation structures have been affected by permanent human pressure on natural resources and the preservation status of the collection sites. Based on our results, we conclude that the investigated areas were probably shelter zones for wild grapevine preservation during the unfavorable glaciation era.
中文翻译:
野生葡萄的评估(葡萄亚种樟子松)chlorotypes并伴随着东部亚得里亚海地区木本植物
东部亚得里亚海地区,涵盖克罗地亚和波斯尼亚和黑塞哥维那,被认为是野生葡萄的自然种群(的一个重要领域葡萄亚种樟子松)。野生葡萄藤出现在亚得里亚海东部地区,在欧盟-地中海和地中海南部的一个接触带,以典型的喀斯特地貌为特征。这项研究的重点是野生葡萄藤的叶绿体DNA(cpDNA)分析以及伴随的木本物种的生物多样性,以更好地了解东亚得里亚海地区樟子松种群的遗传变异,并调查这种变异如何适应当今欧洲野生葡萄藤的分布。欧洲大陆。使用野生个体的9个cpDNA微卫星基因座上的等位基因变异来表征来自四个种群位点的53个个体的单倍型多样性。所有个体都分为两种氯型:A和D,D是欧洲大陆上野生种群中罕见的单倍型。总共,确定了52种木本植物物种。然而,所研究的植被结构受到人类对自然资源的永久压力和收集地点的保存状况的影响。根据我们的结果,我们得出结论,在不利的冰河时代,所调查的地区可能是野生葡萄保护区的避难所。