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The complex global response to copper in the multicellular bacterium Myxococcus xanthus
Metallomics ( IF 3.4 ) Pub Date : 2018-06-21 00:00:00 , DOI: 10.1039/c8mt00121a
Juana Pérez 1, 2, 3, 4, 5 , José Muñoz-Dorado 1, 2, 3, 4, 5 , Aurelio Moraleda-Muñoz 1, 2, 3, 4, 5
Affiliation  

The complex copper response of the multicellular proteobacterium M. xanthus includes structural genes similar to those described in other bacteria, such as P1B-type ATPases, multicopper oxidases, and heavy metal efflux systems. However, the two time-dependent expression profiles of the different copper systems are unique. There are a number of genes responsible for an immediate response, whose expression increases after the addition of copper, but rapidly decreases thereafter to basal levels. The regulatory element that controls this early response is CorE, a novel extracytoplasmic function σ factor that is activated by Cu2+ and inactivated by Cu+. Other genes are part of a maintenance response. These genes show a profile that slows up after the copper addition and reaches a plateau at 24–48 h incubation. Most of the genes involved in this response are encoded by the operon curA, which is regulated by the two-component system CorSR. Moreover, other genes involved in the maintenance response are regulated by different regulatory elements that remain unknown. Additionally, copper activates the transcription of the structural genes for carotenoid synthesis through a mechanism that requires the activation of the σ factor CarQ. Bearing in mind that M. xanthus is not very resistant to copper, it is speculated that the complexity of its copper response might be related to its complex life cycle.

中文翻译:

多细胞细菌黄单球菌对铜的复杂全局响应

多细胞变形菌的复合铜响应黄色粘球菌包括类似于那些在其它细菌,如P描述的结构基因1B型ATP酶,多铜氧化酶,和重金属外排系统。但是,不同铜系统的两个时间相关的表达谱是唯一的。有许多基因可引起立即反应,添加铜后其表达增加,但此后迅速下降至基础水平。所述调控元件控制该早期反应是核心,由铜活化的新颖的细胞质外功能σ因子2+和用Cu灭活+。其他基因是维持反应的一部分。这些基因显示出在添加铜后减慢并在24-48 h孵化达到平稳的过程。参与此反应的大多数基因由操纵子curA编码,该操纵子由两组分系统CorSR调节。而且,参与维持应答的其他基因由未知的不同调控元件调控。此外,铜通过需要激活σ因子CarQ的机制激活类胡萝卜素合成的结构基因的转录。牢记黄腐分支杆菌对铜的抗性不是很强,因此推测其铜响应的复杂性可能与其复杂的生命周期有关。
更新日期:2018-06-21
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