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Molecular mechanism of composite nanoparticles TiO2/WO3/GO-induced activity changes of catalase and superoxide dismutase
Chemico-Biological Interactions ( IF 5.1 ) Pub Date : 2018-06-21 , DOI: 10.1016/j.cbi.2018.06.025
Xiaoyan Hao , Li Zhang , Xin Zheng , Wansong Zong , Chunguang Liu

More and more composite nano-photocatalysts were developed by doping, modifying and coupling, which expanded its application but resulted in pollution due to the unrecyclability. Composite photocatalyst TiO2/WO3/GO, as a model, was evaluated by exploring the molecular mechanism of TiO2/WO3/GO-induced activity changes of catalase (CAT) and superoxide dismutase (SOD). Results showed that TiO2/WO3/GO could lead to conformational and functional changes of CAT and SOD. The activity of both CAT and SOD increased depending on the exposure dose of TiO2/WO3/GO. The change skeleton structure and increase of α-helix content of CAT and SOD were certificated with UV–vis absorption and CD measurements. Intrinsic fluorescence of CAT and SOD were quenched by dynamic quenching. Micro-environment of amino acid residues of CAT and SOD became more hydrophilic, and the microenvironment of Trp residues was more vulnerable than Tyr residues with TiO2/WO3/GO exposure. In addition, inhibitory comparison between GO, TiO2, WO3 and TiO2/WO3/GO was made, results showed that composite nano-photocatalyst exhibited different inhibitory compared to their parent nano-particles.



中文翻译:

复合纳米颗粒TiO 2 / WO 3 / GO诱导过氧化氢酶和超氧化物歧化酶活性变化的分子机理

通过掺杂,改性和偶联开发了越来越多的复合纳米光催化剂,扩大了其应用范围,但由于不可回收性而导致污染。通过探索TiO 2 / WO 3 / GO诱导过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性变化的分子机理,评价了复合光催化剂TiO 2 / WO 3 / GO的模型。结果表明,TiO 2 / WO 3 / GO可能导致CAT和SOD的构象和功能变化。CAT和SOD的活性均随TiO 2 / WO 3的暴露剂量而增加/走。CAT和SOD的骨架结构变化和α-螺旋含量的增加通过UV-vis吸收和CD测量得到证实。CAT和SOD的内在荧光通过动态猝灭来猝灭。暴露于TiO 2 / WO 3 / GO的CAT和SOD氨基酸残基的微环境变得更亲水,并且Trp残基的微环境比Tyr残基更脆弱。另外,对GO,TiO 2,WO 3和TiO 2 / WO 3 / GO的抑制作用进行了比较,结果表明,复合纳米光催化剂与母体纳米颗粒相比具有不同的抑制作用。

更新日期:2018-06-21
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