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Influence of fluoride on the mineralization of collagen via the polymer-induced liquid-precursor (PILP) process
Dental Materials ( IF 5 ) Pub Date : 2018-06-21 , DOI: 10.1016/j.dental.2018.06.020
Neha Saxena 1 , Maegan A Cremer 1 , Evan S Dolling 1 , Hamid Nurrohman 2 , Stefan Habelitz 3 , Grayson W Marshall 3 , Laurie B Gower 1
Affiliation  

Objective

The polymer-induced liquid-precursor (PILP) mineralization process has been shown to remineralize artificial dentin lesions to levels consistent with those of native dentin. However, nanoindentation revealed that the moduli of those remineralized lesions were only ∼50% that of native dentin. We hypothesize that this may be due to the PILP process having been previously optimized to obtain high amounts (∼70 wt%) of intrafibrillar crystals, but without sufficient interfibrillar mineral, another significant component of dentin.

Methods

Fluoride was added to the PILP-mineralization of collagen from rat tail tendon at varying concentrations to determine if a better balance of intra- versus inter-fibrillar mineralization could be obtained, as determined by electron microscopy. Nanoindentation was used to determine if fluoridated apatite could improve the mechanical properties of the composites.

Results

Fluoride was successfully incorporated into the PILP-mineralization of rat tail tendon and resulted in collagen-mineral composite systems with the mineral phase of hydroxyapatite containing various levels of fluoridation. As the fluoride concentration increased, the crystals became larger and more rod-like, with an increasing tendency to form on the fibril surfaces rather than the interior. Nanomechanical testing of the mineralized tendons revealed that fluoride addition did not increase modulus over PILP mineralization alone. This likely resulted from the separated nature of collagen fibrils that comprise tendon, which does not provide lateral reinforcement and therefore may not be suited for the compressive loads of nanoindentation.

Significance

This work contributes to the development of minimally invasive approaches to caries treatment by determining if collagen can be functionally mineralized.



中文翻译:

氟化物通过聚合物诱导液体前体 (PILP) 过程对胶原蛋白矿化的影响

客观的

聚合物诱导的液体前体 (PILP) 矿化过程已被证明可以将人造牙本质病变再矿化至与天然牙本质一致的水平。然而,纳米压痕显示,这些再矿化病变的模量仅为天然牙本质的~50%。我们推测,这可能是由于 PILP 工艺先前已经过优化,以获得大量(~70 wt%)的原纤维内晶体,但没有足够的原纤维间矿物质(牙本质的另一个重要成分)。

方法

将不同浓度的氟化物添加到来自大鼠尾腱的胶原蛋白的 PILP 矿化中,以确定是否可以获得纤维内与纤维间矿化的更好平衡,如通过电子显微镜确定的。纳米压痕用于确定氟化磷灰石是否可以改善复合材料的机械性能。

结果

氟化物成功地融入到大鼠尾腱的 PILP 矿化中,形成了胶原蛋白-矿物质复合系统,其中羟基磷灰石矿物相含有不同程度的氟化。随着氟化物浓度的增加,晶体变得更大并且更像棒状,并且越来越倾向于在原纤维表面而不是内部形成。矿化肌腱的纳米力学测试表明,与单独的 PILP 矿化相比,添加氟化物并没有增加模量。这可能是由于构成肌腱的胶原原纤维的分离性质造成的,肌腱不提供横向加固,因此可能不适合纳米压痕的压缩载荷。

意义

这项工作通过确定胶原蛋白是否可以功能性矿化,有助于开发微创龋齿治疗方法。

更新日期:2018-06-21
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