The aim of this study was to isolate and purify anti-obesity peptides from Spirulina platensis protein. Five enzymes (trypsin, alcalase, pepsin, papain and protamex) were used for hydrolysis of the extracted protein. Subsequently, ultrafiltration and gel column chromatography were employed for further purification. The inhibitory effect on pancreatic lipase and 3T3-L1 preadipocytes were evaluated. The results showed that the 3–5 kDa fraction from pepsin digest exhibited good inhibition on lipase (72%), and its chromatographed fractions displayed strong inhibition on 3T3-L1 preadipocytes (72.7–88.1%) but low inhibitory or stimulatory activities on normal liver cells L-O2 (−26.02 to 12.16%). By mass spectrometry, four novel peptides NALKCCHSCPA, LNNPSVCDCDCMMKAAR, NPVWKRK and CANPHELPNK were identified, which exhibited inhibitory effects on 3T3-L1 preadipocytes proliferation (32.29–60.08%). Moreover, NPVWKRK and CANPHELPNK also significantly decreased the accumulation of triglyceride at 600 μg/mL (p < 0.05), up to 23.7% and 19.5%, respectively. This suggests the potential of Spirulina platensis-derived hydrolysates and peptides as anti-obesity ingredients.

这项研究的目的是从螺旋藻中分离和纯化抗肥胖肽蛋白质。五种酶（胰蛋白酶，alcalase，胃蛋白酶，木瓜蛋白酶和Protamex）用于水解提取的蛋白质。随后，将超滤和凝胶柱色谱法用于进一步纯化。评估了对胰腺脂肪酶和3T3-L1前脂肪细胞的抑制作用。结果表明，胃蛋白酶消化液中的3–5 kDa馏分对脂肪酶表现出良好的抑制作用（72％），其色谱馏分对3T3-L1前脂肪细胞表现出强烈的抑制作用（72.7–88.1％），但对正常肝脏的抑制或刺激活性较低电池L-O2（−26.02至12.16％）。通过质谱分析，鉴定出四种新型肽NALKCCHSCPA，LNNPSVCDCDCMMKAAR，NPVWKRK和CANPHELPNK，它们对3T3-L1前脂肪细胞的增殖具有抑制作用（32.29–60.08％）。而且，NPVWKRK和CANPHELPNK还显着降低了600μg/ mL的甘油三酸酯的积累（p <0.05），分别高达23.7％和19.5％。这表明潜在的螺旋藻衍生的水解产物和肽作为抗肥胖成分。