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Molecular basis for the production of cyclic peptides by plant asparaginyl endopeptidases.
Nature Communications ( IF 16.6 ) Pub Date : 2018-06-20 , DOI: 10.1038/s41467-018-04669-9
M. A. Jackson , E. K. Gilding , T. Shafee , K. S. Harris , Q. Kaas , S. Poon , K. Yap , H. Jia , R. Guarino , L. Y. Chan , T. Durek , M. A. Anderson , D. J. Craik

Asparaginyl endopeptidases (AEPs) are proteases that have crucial roles in plant defense and seed storage protein maturation. Select plant AEPs, however, do not function as proteases but as transpeptidases (ligases) catalyzing the intra-molecular ligation of peptide termini, which leads to peptide cyclization. These ligase-type AEPs have potential biotechnological applications ranging from in vitro peptide engineering to plant molecular farming, but the structural features enabling these enzymes to catalyze peptide ligation/cyclization rather than proteolysis are currently unknown. Here, we compare the sequences, structures, and functions of diverse plant AEPs by combining molecular modeling, sequence space analysis, and functional testing in planta. We find that changes within the substrate-binding pocket and an adjacent loop, here named the "marker of ligase activity", together play a key role for AEP ligase efficiency. Identification of these structural determinants may facilitate the discovery of more ligase-type AEPs and the engineering of AEPs with tailored catalytic properties.

中文翻译:

植物天冬酰胺基内肽酶生产环状肽的分子基础。

天冬酰胺基内肽酶(AEP)是在植物防御和种子贮藏蛋白成熟中具有关键作用的蛋白酶。但是,精选的植物AEP并不充当蛋白酶,而是作为催化肽末端分子内连接的转肽酶(连接酶),从而导致肽环化。这些连接酶类型的AEP具有潜在的生物技术应用范围,从体外肽工程到植物分子耕种,但是使这些酶催化肽的连接/环化而不是蛋白水解的结构特征目前尚不清楚。在这里,我们通过结合分子建模,序列空间分析和在植物中的功能测试,比较了多种植物AEP的序列,结构和功能。我们发现底物结合口袋和相邻环内的变化,此处被称为“连接酶活性标记”,共同对AEP连接酶效率起关键作用。这些结构决定因素的鉴定可能有助于发现更多的连接酶型AEP和具有定制催化特性的AEP工程。
更新日期:2018-06-20
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