This study assesses the effects of cyclic fatty acid monomers (CFAM) from heated vegetable oils on oxidative stress and inflammation. Wistar rats were fed either of these four diets for 28 days: canola oil (CO), canola oil and 0.5% CFAM (CC), soybean oil (SO), and soybean oil and 0.5% CFAM (SC). Markers of oxidative stress and inflammation were determined by micro liquid chromatography tandem mass spectrometry (micro-LC–MS/MS) and enzyme-linked immunosorbent assay (ELISA) kits, respectively. Analysis of variance (ANOVA) for a 2 × 2 factorial design was performed to determine the CFAM and oil effects and interactions between these two factors at P ≤ 0.05. For significant interactions, a post hoc multiple comparison test was performed, i.e., Tukey HSD (honest significant difference) test. CFAM induced higher plasma levels of 15-F_2t-IsoP (CC, 396 ± 43 ng/mL, SC, 465 ± 75 ng/mL vs CO, 261 ± 23 ng/mL and SO, 288 ± 35 ng/mL, P < 0.05). Rats fed the SC diet had higher plasma 2,3-dinor-15-F_2t-IsoP (SC, 145 ± 9 ng/mL vs CC, 84 ± 8 ng/mL, CO, 12 ± 1 ng/mL, and SO, 12 ± 1 ng/mL, P < 0.05), urinary 2,3-dinor-15-F_2t-IsoP (SC, 117 ± 12 ng/mL vs CC, 67 ± 13 ng/mL, CO, 15 ± 2 ng/mL, and SO, 18 ± 4 ng/mL, P < 0.05), and plasma IL-6 (SC, 57 ± 10 pg/mL vs CC, 48 ± 11 pg/mL, CO, 46 ± 9 pg/mL, and SO, 44 ± 4 pg/mL, P < 0.05) than the other three diet groups. These results indicate that CFAM increased the levels of markers of oxidative stress, and those effects are exacerbated by a CFAM–high-linoleic acid diet.

中文翻译：

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加热植物油中的环状脂肪酸单体对雄性Wistar大鼠炎症和氧化应激指标的影响

这项研究评估了加热的植物油中的环状脂肪酸单体（CFAM）对氧化应激和炎症的影响。向Wistar大鼠喂食这四种饮食中的一种，持续28天：低芥酸菜子油（CO），低芥酸菜子油和0.5％CFAM（CC），大豆油（SO）和大豆油和0.5％CFAM（SC）。分别通过微型液相色谱串联质谱（micro-LC-MS / MS）和酶联免疫吸附测定（ELISA）试剂盒确定氧化应激和炎症的标志物。进行了2×2因子设计的方差分析（ANOVA），以确定CFAM和油的影响以及这两个因素在P处的相互作用。≤0.05。对于重要的交互作用，执行事后多重比较测试，即Tukey HSD（诚实显着差异）测试。CFAM诱导的15-F _2t -IsoP血浆水平更高（CC，396±43 ng / mL，SC，465±75 ng / mL vs CO，261±23 ng / mL和SO，288±35 ng / mL，P <0.05）。饲喂SC饮食的大鼠血浆2,3-dinor-15-F _2t -IsoP较高（SC为145±9 ng / mL，而CC为84±8 ng / mL，CO，12±1 ng / mL和SO ，12±1 ng / mL，P <0.05），尿液2,3-dinor-15-F _2t -IsoP（SC，117±12 ng / mL vs CC，67±13 ng / mL，CO，15±2 ng / mL和SO，18±4 ng / mL，P <0.05）和血浆IL-6（SC，57±10 pg / mL vs CC，48±11 pg / mL，CO，46±9 pg / mL mL和SO，44±4 pg / mL，P<0.05）高于其他三个饮食组。这些结果表明，CFAM增加了氧化应激标志物的水平，而CFAM-高亚油酸饮食则加剧了这些影响。