New peptides derived from the natural antimicrobial temporin B were obtained. The design, antimicrobial activity, and characterization of the secondary structure of peptides in the presence of bacterial cells is described herein. TB_KKG6K (KKLLPIVKNLLKSLL) has been identified as the most active analogue against Gram‐positive and ‐negative bacteria, compared with natural temporin B (LLPIVGNLLKSLL) and TB_KKG6A (KKLLPIVANLLKSLL). Acylation with hydrophobic moieties generally led to reduced activity; however, acylation at the 6‐position of TB_KKG6K led to retained sub‐micromolar activity against Staphylococcus epidermidis.

中文翻译：

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酰化对Temporin B类似物抗菌活性的影响

获得了衍生自天然抗微生物temporin B的新肽。本文描述了在细菌细胞存在下肽的二级结构的设计，抗微生物活性和表征。与天然temporin B（LLPIVGNLLKSLL）和TB_KKG6A（KKLLPIVANLLKSLL）相比，TB_KKG6K（KKLLPIVKNLLKSLL）被确定为对革兰氏阳性和阴性细菌活性最高的类似物。与疏水性部分酰化通常导致活性降低；然而，在TB_KKG6K的6位酰化导致保留了对表皮葡萄球菌的亚微摩尔活性。