A new molecularly imprinted polymer electrochemiluminescence (ECL) sensor was developed for the detection of clopyralid (CPD) based on enzyme-biocatalyzed amplification. CdTe quantum dots were immobilized on the surface of an electrode by reaction with p-aminothiophenol preadsorbed on the electrode. Then a molecularly imprinted film was formed by electrochemical polymerization of o-phenylenediamine in the presence of CPD on the CdTe-modified gold electrode. During the analytical cycle, horseradish peroxidase (HRP)-labeled CPD was replaced by CPD in the sample. The amount of HRP on the molecularly imprinted polymer electrode decreased, and then less H₂O₂ was catalytically decomposed. Subsequently, the ECL intensity of the CdTe–H₂O₂ system was enhanced. There was a good linear relationship between ECL intensity and the concentration of CPD in the range from 2.0 × 10^-11 to 2.5 × 10^-10 mol/L and in the range from 2.5 × 10^-10 to 3.5 × 10^-8 mol/L. The detection limit was 4.1 × 10^-12 mol/L. The sensor was applied to determine CPD in vegetable samples.

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开发了一种新的分子印迹聚合物电化学发光（ECL）传感器，用于基于酶生物催化扩增的氯吡格雷（CPD）检测。通过与预吸附在电极上的对氨基硫酚反应，将CdTe量子点固定在电极表面上。然后，在CPD存在下，在CPD存在下，通过邻苯二胺在CPD存在下进行电化学聚合，形成分子印迹膜。在分析周期中，样品中的CPD代替了辣根过氧化物酶（HRP）标记的CPD。分子印迹聚合物电极上的HRP量减少，然后更少的H ₂ O ₂被催化分解。随后，CdTe–H的ECL强度₂ O ₂系统得到了增强。ECL强度与CPD浓度在2.0×10 ^-11至2.5×10 ^-10 mol / L以及2.5×10 ^-10至3.5×10 ^-8 mol / L的范围内具有良好的线性关系。L. 检出限为4.1×10 ^-12 mol / L。该传感器用于确定蔬菜样品中的CPD。

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