The host−guest interactions between Auramine O (AuO) dye and cucurbit[n]uril (CB[n], n = 7, 8) were investigated in aqueous solution by ¹H NMR, UV/Vis, fluorescence spectroscopic techniques and single crystal diffraction. The results indicate the simultaneous formation of 1:1 and 2:1 inclusion complexes between CB[7] and AuO with two binding constants as 1.17 × 10⁵ M⁻¹ and 3.99 × 10² M⁻¹ by UV/Vis spectroscopy titration (1.20 × 10⁵ M⁻¹ and 5.26 × 10³ M⁻¹ by fluorescence spectroscopy) in phosphate buffer (pH 7.4). And a 1:2 homoternary inclusion complex CB[8]·AuO₂ with binding constant as 2.95 × 10⁹ M⁻² (by fluorescence spectroscopy titration) is detected. Interestingly, the appearance of intense new broad emission band with big red-shift (100 nm) with respect to that of AuO proves the formation of excimer upon encapsulation of AuO molecules in CB[8]. Determination of single crystal structure of CB[8]·AuO₂ further discloses the existence of J-type dimer with intermolecular CH···π interactions between two included phenyl groups on AuO molecules. Upon host−guest complexation of AuO with CB[7] or CB[8], the pK_a shifts of AuO were measured.

金胺O（之间的主-客体相互作用AUO）染料和葫芦[ Ñ ]脲（CB [ Ñ ]，Ñ  = 7,8）通过在水溶液中进行了研究¹ H NMR，UV /可见光谱，荧光光谱技术和单晶衍射。结果表明，通过UV / Vis光谱滴定法，CB [7]与AuO同时形成了1：1和2：1的包合物，两个结合常数分别为1.17×10 ⁵  M ^-1和3.99×10 ²  M ^-1（ 1.20×10 ⁵  M ^-1和5.26×10 ³  M ^-1在磷酸盐缓冲液（pH 7.4）中通过荧光光谱法）。并检测出结合常数为2.95×10 ⁹  M ^-2的1：2均聚物包合物CB [8]· AuO ₂（荧光光谱滴定法）。有趣的是，相对于AuO而言，强红移（100 nm）的强新宽发射带的出现证明了将AuO分子包裹在CB中会形成准分子[8]。CB [8]· AuO ₂单晶结构的确定进一步公开了在AuO分子上两个包含的苯基之间具有分子间CH···π相互作用的J型二聚体的存在。在主客复合体友达与CB [7]或CB [8]中，p ķ_一个的位移友达进行测定。