We developed EASI-tag (easily abstractable sulfoxide-based isobaric-tag), a new type of amine-derivatizing and sulfoxide-containing isobaric labeling reagents for highly accurate quantitative proteomics analysis using mass spectrometry. We observed that EASI-tag labels dissociate at low collision energy and generate peptide-coupled, interference-free reporter ions with high yield. Efficient isolation of ¹²C precursors and quantification at the MS2 level allowed accurate determination of quantitative differences between up to six multiplexed samples.

中文翻译：

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EASI标签可实现准确的多路复用且无干扰的基于MS2的蛋白质组定量

我们开发了EASI标签（可轻松提取的基于亚砜的同量异位标记），这是一种新型的胺衍生化和含亚砜的同量异位标记试剂，可使用质谱进行高精度的定量蛋白质组学分析。我们观察到，EASI标签标签在低碰撞能量下会解离，并以高收率产生肽偶联的，无干扰的报告离子。有效分离¹² C前体并在MS2级别进行定量，可以准确测定多达六个多重样品之间的定量差异。