Peroxynitrite (ONOO⁻) is an extremely powerful biological oxidant and it can react with a wide variety of molecular targets to result in a series of disease states, which has made ONOO⁻ a central biological pathogenic factor. In order to disclose its various pathological events, a large number of fluorescent ONOO⁻ probes have been developed in recent years. Nevertheless, some limitations still remain, such as short excitation and emission wavelengths, long fluorescence response times, and cross-interference caused by other reactive oxygen species (ROS). In this work, a new near-infrared (NIR) fluorescent probe, SiRTA, containing an aromatic tertiary amine functional group and a Si-rhodamine fluorophore, was developed for endogenous ONOO⁻ detection and imaging. The probe exhibits not only a fast, sensitive, and specific fluorescence off–on response toward ONOO⁻ in chemical systems but also excellent imaging ability for endogenous ONOO⁻ in living cells. With the probe, the therapeutic effects of phenolic acid antioxidants in EA.hy926 endothelial cells suffering from ischemia–reperfusion injury and the pathogenesis of diabetes and diabetic nephropathy in pancreatic β-cells and diabetic rats have successfully been evaluated. These results indicate that SiRTA is a potentially outstanding imaging tool for studying the physiological and pathological events of ONOO⁻ and relevant drug therapies.

中文翻译：

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基于罗丹明的近红外荧光探针，用于观察活细胞，组织和动物中的内源性过氧亚硝酸盐† ‡

过氧亚硝酸盐（ONOO ^-导致一系列疾病状态，这已ONOO制成的是一个非常强大的生物氧化剂，它可以与各种各样的分子靶标反应）^-中央生物的致病因子。为了揭示它的各种病理事件，大量荧光ONOO的^-探测已经发展在最近几年。但是，仍然存在一些局限性，例如激发和发射波长短，荧光响应时间长以及其他活性氧（ROS）引起的交叉干扰。在这项工作中，一种新的近红外（NIR）荧光探针SiRTA含有芳香族叔胺官能团和Si-若丹明荧光团，被内源性ONOO开发^-检测和成像。探针表现出不仅是一种快速，灵敏，和特异性荧光关-开响应朝向ONOO ^-在化学系统也为内源性ONOO极好的成像能力^-在活细胞。用该探针成功地评估了酚酸抗氧化剂在EA.hy926内皮细胞缺血再灌注损伤以及胰腺β细胞和糖尿病大鼠中糖尿病和糖尿病肾病的发病机理中的治疗作用。这些结果表明，SiRTA是研究ONOO的生理和病理事件潜在的优秀成像工具^-与相关的药物治疗。