Our article presents the development and validation of two simple, very sensitive, and low-cost spectroscopic methods for the assay of milnacipran hydrochloride in bulk form, pharmaceutical tablets and spiked human urine and plasma. Spectroscopic methods (spectrophotometric and spectrofluorimetric techniques) were dependent on the chromogenic and fluorogenic properties of the 4-chloro-7 nitrobenzofurazan (NBD-Cl) reagent. The reaction product, resulting from the interaction between NBD-Cl and milnacipran in the presence of borate buffer pH 8.5, was measured spectrophotometrically at 465 nm and spectrofluorimetrically at 510 nm after excitation at 465 nm. The absorbance–concentration plot was rectilinear over the range of 1.5–12 μg mL⁻¹ with a limit of quantitation 1.09 μg mL⁻¹, while the fluorescence–concentration plot was rectilinear over the range of 0.03–0.5 μg mL⁻¹ with a limit of quantitation 0.02 μg mL⁻¹. Influential parameters affecting the development and stability of the reaction product were studied and optimized. Assurance of the cited drug in its tablets by our proposed methods was successfully completed without obstruction from the presence of the basic excipients with average percentage recoveries of 99.27 ± 1.18 and 99.44 ± 0.69 for the spectrophotometric and spectrofluorimetric methods, respectively. The spectrofluorimetric method was additionally adopted as a preliminary in vitro study for the assay of the cited drug in spiked human urine and plasma with average percentage recoveries of 101.52 ± 1.01 and 100.38 ± 1.57 for spiked urine and plasma, respectively.

中文翻译：

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苯并呋喃的显色和荧光特性在检测人尿液和血浆中的米那普仑时的效用†

我们的文章介绍了两种简单、非常灵敏和低成本的光谱方法的开发和验证，用于分析散装形式的盐酸米那普仑、药片和加标的人尿和血浆。光谱方法（分光光度法和分光荧光法）取决于 4-chloro-7 nitrobenzofuraza (NBD-Cl) 试剂的显色和荧光特性。在硼酸盐缓冲液 pH 8.5 存在下，由 NBD-Cl 和米那普仑之间的相互作用产生的反应产物在 465 nm 分光光度计和 465 nm 激发后在 510 nm 分光荧光法测量。吸光度-浓度曲线在 1.5-12 μg mL ^-1范围内呈直线，定量限为 1.09 μg mL ^-1，而荧光浓度曲线在 0.03-0.5 μg mL ^-1范围内呈直线，定量限为 0.02 μg mL ^-1。对影响反应产物发展和稳定性的影响参数进行了研究和优化。通过我们提出的方法成功地完成了对片剂中引用药物的保证，没有受到基本赋形剂存在的阻碍，分光光度法和分光荧光法的平均回收率分别为 99.27 ± 1.18 和 99.44 ± 0.69。另外采用荧光分光光度法作为体外初步对加标人尿液和血浆中引用的药物进行测定的研究，加标尿液和血浆的平均回收率分别为 101.52 ± 1.01 和 100.38 ± 1.57。