The development of multifunctional nanoplatform, which integrates biosensing and imaging diagnosis, for accurate identification of cancer cells in blood or other bodily fluids is critical for early cancer diagnosis and treatment. Herein, by using a facile single spinneret electrospinning technique followed by thermal treatment, CuO-NiO/C heterojunction nanofiber was synthesized. The resultant CuO-NiO/C nanofibers exhibited excellent catalytic activity toward ammonia borane (AB) hydrolysis, producing a marked pressure increase in a closed reaction vessel. Moreover, the resultant nanofibers showed highly efficient catalytic activity for reduction of rhodamine 6G (Rh6G, fluorescent molecule), accompanied by the fluorescence quenching. On the basis of these findings, a novel sensing platform utilizing folic-acid (FA) conjugated CuO-NiO/C nanofibers as an artificial enzyme and recognition element for fluorescence imaging and pressure-based (pressure meter) detection of folate receptor (FR)-positive cancer cells was developed. The detection limit is as low as 50 cells/mL. What’s more, the clinical applicability of the sensor was also proven to be suitable for the sensing cancer cells in whole blood. The most important advantage of the synthesized nanofibers based-probe is the combination of pressure meter and fluorescence imaging to specifically see and sensitively count the cancer cells, paving a new way in early liver cancer diagnosis.

中文翻译：

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基于压力和荧光双信号读出的基于CuO-NiO / C异质结纳米纤维的纳米平台用于血液中靶癌细胞的成像和检测

集成生物传感和影像诊断功能的多功能纳米平台的开发，用于准确识别血液或其他体液中的癌细胞对于早期癌症的诊断和治疗至关重要。在此，通过使用简单的单喷丝头电纺丝技术然后进行热处理，合成了CuO-NiO / C异质结纳米纤维。所得的CuO-NiO / C纳米纤维对氨硼烷（AB）的水解表现出优异的催化活性，在密闭的反应容器中产生显着的压力升高。此外，所得的纳米纤维显示出用于还原若丹明6G（Rh6G，荧光分子）的高效催化活性，并伴随着荧光猝灭。根据这些发现，开发了一种利用叶酸（FA）共轭的CuO-NiO / C纳米纤维作为人工酶和识别元件的新型传感平台，用于荧光成像和基于压力（压力计）检测叶酸受体（FR）阳性癌细胞。检出限低至50细胞/ mL。此外，该传感器的临床适用性也被证明适用于感测全血中的癌细胞。合成的基于纳米纤维的探针的最重要的优势是压力计和荧光成像的结合，可以特异性地观察和敏感地计数癌细胞，为早期肝癌诊断铺平了新的道路。检出限低至50细胞/ mL。此外，该传感器的临床适用性也被证明适用于感测全血中的癌细胞。合成的基于纳米纤维的探针的最重要的优势是压力计和荧光成像的结合，可以特异性地观察和敏感地计数癌细胞，为早期肝癌诊断铺平了新的道路。检出限低至50细胞/ mL。此外，该传感器的临床适用性也被证明适用于感测全血中的癌细胞。合成的基于纳米纤维的探针的最重要的优势是压力计和荧光成像的结合，可以特异性地观察和敏感地计数癌细胞，为早期肝癌诊断铺平了新的道路。