Probes for monitoring aggregation of amyloid beta (Aβ) peptides are crucial to advance understanding of the molecular pathogenesis of Alzheimer's Disease (AD). Here, we report luminescent tricarbonyl rhenium complexes of tetraarylethylene (TAE) ligands featuring bis(benzothiazole) chelating groups in combination with (oligo)thiophene units that have been designed for monitoring amyloid fibrillation. Variation in the number of thiophenes influenced the photophysical properties of these complexes, as well as their binding affinities toward Aβ₄₂ fibrils. All complexes displayed submicromolar K_d's for binding Aβ₄₂ aggregates accompanied by up to 34‐fold enhanced luminescence and red‐shifted emission wavelengths. The high binding affinities and desirable photophysical properties of these complexes render them potential alternatives to established fluorescent Aβ probes such as thioflavin T. Additionally, the general and modular design approach implemented in this study should facilitate development of second‐generation TAE‐based diagnostic tools for studying protein aggregation in AD and other neurological diseases.

中文翻译：

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双（苯并噻唑）基四芳基乙烯的hen配合物作为淀粉样蛋白纤维的选择性发光探针。

监测淀粉样β（Aβ）肽聚集的探针对于增进对阿尔茨海默氏病（AD）分子发病机制的了解至关重要。在这里，我们报告四芳基乙烯（TAE）配体具有双（苯并噻唑）螯合基团与（寡聚）噻吩单元组合的发光三羰基rh配合物，该配合物已设计用于监测淀粉样蛋白原纤化。噻吩数量的变化影响了这些复合物的光物理性质，以及它们对_Aβ42原纤维的结合亲和力。所有复合物均显示亚微摩尔K _d与Aβ42结合聚集体具有高达34倍的增强发光和红移发射波长。这些复合物的高结合亲和力和理想的光物理性质使其成为已建立的荧光Aβ探针（如thioflavin T）的潜在替代物。此外，本研究中采用的通用和模块化设计方法应有助于开发基于第二代TAE的诊断工具研究AD和其他神经系统疾病中的蛋白质聚集。