Sea squirt (Halocynthia roretzi) shell protein (SSP) was used as a biodegradable film material and laminated with polylactic acid (PLA) to improve its physical and water barrier properties. Cinnamon bark oil (CBO) was incorporated into the SSP film as a bioactive material. After laminating with PLA, the tensile strength and elongation at break of the SSP film increased from 4.07 to 9.09 MPa and from 8.68 to 138.84%, respectively. In addition, water vapor permeability and water solubility decreased from 5.62 to 0.91 × 10-9 g m/m2 s Pa and from 42.17% to 23.93%, respectively. DSC results of the SSP films indicate that melting point temperature increased 140.05 to 163.52 °C by laminating PLA. The addition of 0.5%, 0.7%, and 1.0% CBO conferred the antimicrobial activity against four pathogenic bacteria to the SSP/PLA-laminated films. The SSP/PLA-laminated films containing CBO also had antioxidant activities. Therefore, the SSP/PLA-laminated films containing CBO are applicable as biodegradable packaging films. PRACTICAL APPLICATION Sea squirt shell has been discarded after the consumption of sea squirt, and sea squirt shell protein can be a base material for biodegradable films. In this study, sea squirt shell protein and polylactic acid laminated films containing cinnamon bark essential oil were developed. The developed films are promising environmentally-friendly alternatives for active packaging material.

中文翻译：

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含有肉桂皮精油的海鞘蛋白和聚乳酸复合薄膜

海鞘（Halocynthia roretzi）壳蛋白（SSP）被用作可生物降解的薄膜材料，并与聚乳酸（PLA）层压以提高其物理和防水性能。肉桂皮油 (CBO) 作为生物活性材料被加入到 SSP 薄膜中。与PLA复合后，SSP薄膜的拉伸强度和断裂伸长率分别从4.07 MPa提高到9.09 MPa，从8.68提高到138.84%。此外，水汽渗透率和水溶性分别从 5.62 下降到 0.91 × 10-9 gm/m2 s Pa 和从 42.17% 下降到 23.93%。SSP 薄膜的 DSC 结果表明，通过层压 PLA，熔点温度提高了 140.05 至 163.52 °C。添加 0.5%、0.7% 和 1.0% CBO 赋予 SSP/PLA 层压薄膜对四种病原菌的抗菌活性。含有 CBO 的 SSP/PLA 复合薄膜也具有抗氧化活性。因此，含有 CBO 的 SSP/PLA 复合薄膜可用作可生物降解的包装薄膜。实际应用 海鞘在食用后被丢弃，海鞘蛋白可作为生物降解薄膜的基材。在这项研究中，开发了含有肉桂皮精油的海鞘蛋白和聚乳酸层压薄膜。开发的薄膜是有前途的活性包装材料的环保替代品。海鞘蛋白可以作为生物降解薄膜的基材。在这项研究中，开发了含有肉桂皮精油的海鞘蛋白和聚乳酸层压薄膜。开发的薄膜是有前途的活性包装材料的环保替代品。海鞘蛋白可以作为生物降解薄膜的基材。在这项研究中，开发了含有肉桂皮精油的海鞘蛋白和聚乳酸层压薄膜。开发的薄膜是有前途的活性包装材料的环保替代品。