当前位置: X-MOL 学术J. Food Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Immunoreactivity of Biochemically Purified Amandin from Thermally Processed Almonds (Prunus dulcis L.)
Journal of Food Science ( IF 3.9 ) Pub Date : 2018-06-15 , DOI: 10.1111/1750-3841.14206
Valerie D Zaffran 1 , Shridhar K Sathe 1
Affiliation  

Almond seeds were subjected to select thermal processing and amandin was purified from processed and unprocessed (control) seeds using cryoprecipitation. Amandin immunoreactivity was assessed using two murine monoclonal antibodies (mAbs)-4C10 and 4F10 detecting human IgE-relevant conformational and linear epitopes, respectively. Overall amandin immunoreactivity following thermal treatment ranged from 64.9% to 277.8% (4C10) and 81.3% to 270.3% (4F10). Except for autoclaving (121 °C, 15 psi, 30 min) and roasting (160 °C, 30 min), the tested processing conditions resulted in increased immunoreactivity as determined by mAbs 4C10 and 4F10-based enzyme-linked immunosorbent assays (ELISAs). A significant, yet not complete, reduction in immunoreactivity was caused by autoclaving (121 °C, 15 psi, 30 min) and roasting (160 °C, 30 min). Western- and dot-blot immunoassays corroborated the ELISA results, confirming amandin thermal stability. PRACTICAL APPLICATION The tested immunoassays indicated amandin to be stable, regardless of the targeted epitope and the processing method that whole almond seeds were subjected to.

中文翻译:

来自热处理杏仁 (Prunus dulcis L.) 的生化纯化 Amandin 的免疫反应性

对杏仁种子进行选择性热处理,并使用冷沉淀从加工和未加工(对照)种子中纯化阿曼丁。使用分别检测人 IgE 相关构象和线性表位的两种鼠单克隆抗体 (mAb)-4C10 和 4F10 评估 Amandin 免疫反应性。热处理后的总体 amandin 免疫反应性范围为 64.9% 至 277.8% (4C10) 和 81.3% 至 270.3% (4F10)。除了高压灭菌(121 °C,15 psi,30 分钟)和烘烤(160 °C,30 分钟)外,测试的加工条件导致免疫反应性增加,如基于 mAb 4C10 和 4F10 的酶联免疫吸附试验 (ELISA) . 高压灭菌(121 °C,15 psi,30 分钟)和烘烤(160 °C,30 分钟)导致免疫反应性显着但不完全降低。Western-和点印迹免疫测定证实了ELISA结果,证实了amandin的热稳定性。实际应用 所测试的免疫测定表明,amandin 是稳定的,与整个杏仁种子所经历的靶向表位和加工方法无关。
更新日期:2018-06-15
down
wechat
bug