Patulin (PAT), deoxynivalenol (DON) and toxin T-2 (T-2) are mycotoxins distributed worldwide in food and feed. Cytotoxicity of the three mycotoxins individually or in combination in human hepatocellular carcinoma (HepG2) cells was evaluated by MTT assay over 24, 48 and 72 h of exposure. The concentration ranges used were 0.625–15 μM for DON, 1.25–50 nM for T-2 and 0.45–7.5 μM for PAT. The IC₅₀ values obtained ranged from 9.30 to 2.53 μM, from 33.69 to 44.37 nM and from 2.66 to 1.17 μM for DON, T-2 and PAT, respectively. The most cytotoxic mycotoxin to HepG2 cells was T-2 followed by PAT and DON. The combination ratios used for the mixtures were 1:3 (DON: T-2), 1:5 (DON: PAT), 1:1.7 (T-2: PAT) and 1:3:5 (DON: T-2: PAT). The mixture with the highest cytotoxic effect was T-2+PAT, followed by DON + T-2+PAT, DON + T-2 and DON + PAT respect to the cytotoxic effect of their individuals. In the combinations, at low fa an antagonistic effect was detected, and this effect changes the shape of the combination to additive effect at high fa in the mixtures.

棒曲霉素（PAT），脱氧雪腐酚（DON）和毒素T-2（T-2）是分布在全球食品和饲料中的霉菌毒素。在暴露的24、48和72小时内，通过MTT分析评估了三种真菌毒素在人肝细胞癌（HepG2）细胞中的细胞毒性。DON的浓度范围为0.625–15μM，T-2的浓度范围为1.25–50 nM，PAT的浓度范围为0.45–7.5μM。IC ₅₀对于DON，T-2和PAT，获得的值分别为9.30至2.53μM，33.69至44.37 nM和2.66至1.17μM。对HepG2细胞最具细胞毒性的霉菌毒素是T-2，其次是PAT和DON。用于混合物的混合比例为1：3（DON：T-2），1：5（DON：PAT），1：1.7（T-2：PAT）和1：3：5（DON：T-2） ：PAT）。具有最高细胞毒性作用的混合物为T-2 + PAT，其个体的细胞毒性作用依次为DON + T-2 + PAT，DON + T-2和DON + PAT。在组合物中，在低fa时检测到拮抗作用，这种作用将组合物的形状改变为混合物在高fa时的加和作用。