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Hardy kiwi leaves extracted by multi-frequency multimode modulated technology: A sustainable and promising by-product for industry
Food Research International ( IF 8.1 ) Pub Date : 2018-06-15 , DOI: 10.1016/j.foodres.2018.06.029
Federica Marangi , Diana Pinto , Lizziane de Francisco , Rita C. Alves , Hélder Puga , Stefania Sut , Stefano Dall'Acqua , Francisca Rodrigues , M. Beatriz P.P. Oliveira

The aim of this study is to evaluate hardy kiwi (Actinidia arguta L.) leaves, a crop waste, as a source of bioactive compounds with potential application in different industries, such as food industry. For this study, a new, green and sustainable extraction technique was employed: Multi-Frequency Multimode Modulated Technology. The total phenolic and flavonoid contents (TPC and TFC, respectively) as well as the antioxidant activity (through DPPH and FRAP assays) and the scavenging capacity against reactive species (O2·-, H2O2, NO·, ROO·, HOCl and ONOO·) were evaluated. The cell viability effects were assessed on keratinocytes and fibroblasts and the phytochemical profile was stablished by HPLC-DAD-EIS-MS.

The extract displayed a high antioxidant activity (IC50 = 270.17 ± 69.94 μg/mL through scavenging activity on DPPH radicals and 3219.55 ± 34.29 μmol Fe2+/g dm in the FRAP assay). The TPC was higher than the one reported for fruit. On the contrary, the TFC is lower. Concerning the scavenging capacity against reactive species, the extract was more effective against O2·- and H2O2. Regarding the cell viability assays, the extract did not affect the cell viability on HaCaT and HFF cells, in concentrations below 500 μg/mL and 125 μg/mL, respectively. The HPLC-DAD-EIS-MS-based phytochemical profile reported the presence of chlorogenic acid and glycosylated quercetin as well as kaempferol derivatives.

Overall, the present results suggest that A. arguta leaves are a promising by-product when extracted by this innovative technology, being a valuable source of bioactive compounds for different industries, particularly food industry.



中文翻译:

通过多频多模调制技术提取的强硬猕猴桃叶子:一种可持续且有希望的工业副产品

这项研究的目的是评估作物废料-猕猴桃(Actinidia arguta L.)叶作为生物活性化合物的来源,并可能在食品工业等不同行业中得到应用。在这项研究中,采用了一种新的,绿色的,可持续的提取技术:多频多模调制技术。总酚和类黄酮含量(分别为TPC和TFC)以及抗氧化剂活性(通过DPPH和FRAP分析)和对活性物质(O2 ·-,H 2 O 2,NO ·,ROO ·,HOCl的清除能力)和ONOO ·)进行了评估。评价细胞活力对角质形成细胞和成纤维细胞的影响,并通过HPLC-DAD-EIS-MS建立植物化学特征。

提取物显示出高抗氧化活性( 通过对DPPH自由基的清除活性和FRAP分析中的3219.55±34.29μmolFe 2+ / g dm清除活性,IC 50 = 270.17±69.94μg/ mL )。TPC高于报道的TPC。相反,TFC较低。关于对活性物质的清除能力,提取物对O 2 ·-和H 2 O 2更有效。关于细胞生存力测定,浓度分别低于500μg/ mL和125μg/ mL的提取物不影响HaCaT和HFF细胞的细胞生存力。基于HPLC-DAD-EIS-MS的植物化学概况报告了绿原酸和糖基化槲皮素以及山ka酚衍生物的存在。

总的来说,目前的结果表明,当用这种创新技术提取时,紫茎泽兰叶是有希望的副产物,是不同工业特别是食品工业中生物活性化合物的宝贵来源。

更新日期:2018-06-15
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