Titanium (Ti) implants are widely used in the clinic as bone substitutes and dental implants, but further improvements are needed to obtain high osteogenic ability and consequent osseointegration. Knockdown of long noncoding RNA MIR31HG promotes osteogenic differentiation and bone formation. In this study, we fabricated a Ti surface functionalized with siRNA targeting MIR31HG (siMIR31HG) and accelerated osteogenesis of bone marrow mesenchymal stem cells (BMSCs). Chitosan/siRNA complex was loaded onto the thermal alkali-treated Ti surface to fabricate the siMIR31HG-functionalized Ti surface. The surface morphology, siRNA loading and release efficiency, and transfection efficacy were investigated, and the biological effects, such as cell proliferation, cell morphology, and osteogenic activity, were determined. The results showed that the siMIR31HG-functionalized Ti implant generated an ∼50% knockdown of MIR31HG, with no apparent cytotoxicity, which consequently enhanced osteogenic differentiation of BMSCs, as indicated by the increase of ALP production, extracellular matrix mineralization, osteogenic gene expression, and ectopic bone formation in vivo. The siMIR31HG biofunctionalization can be used to obtain better osseointegration of Ti implant in the clinic.

中文翻译：

---

siMIR31HG功能化的钛表面促进骨髓间充质干细胞成骨分化。

钛（Ti）植入物在临床上广泛用作骨替代物和牙科植入物，但是需要进一步的改进以获得高的成骨能力和随之而来的骨整合。抑制长的非编码RNA MIR31HG可以促进成骨细胞分化和骨形成。在这项研究中，我们制造了一个以靶向MIR31HG（siMIR31HG）的siRNA功能化的Ti表面，并加速了骨髓间充质干细胞（BMSCs）的成骨作用。将壳聚糖/ siRNA复合物加载到经过碱处理的热Ti表面上，以制造siMIR31HG-官能化的Ti表面。研究了表面形态，siRNA加载和释放效率以及转染效率，并确定了细胞增殖，细胞形态和成骨活性等生物学效应。结果表明，siMIR31HG功能化的Ti植入物产生了约50％的MIR31HG敲低，没有明显的细胞毒性，因此增强了BMSC的成骨分化，如ALP产量增加，细胞外基质矿化，成骨基因表达和体内异位骨形成。siMIR31HG生物功能化可用于在临床中获得更好的钛植入物骨整合。