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Using porous magnetic iron oxide nanomaterials as a facile photoporation nanoplatform for macromolecular delivery†
Journal of Materials Chemistry B ( IF 7 ) Pub Date : 2018-06-14 00:00:00 , DOI: 10.1039/c8tb01026a
Lei Wang 1, 2, 3, 4, 5 , Jingxian Wu 1, 2, 3, 4, 5 , Yayun Hu 1, 2, 3, 4, 5 , Changming Hu 1, 2, 3, 4, 5 , Yue Pan 1, 2, 3, 4, 5 , Qian Yu 1, 2, 3, 4, 5 , Hong Chen 1, 2, 3, 4, 5
Affiliation  

The intracellular delivery of exogenous macromolecules such as functional proteins, antibodies, polysaccharides and nucleic acids into living cells for biomedical applications is of great interest. Even though great efforts have been devoted to this task, universal delivery systems that provide excellent intracellular delivery performance combined with easy cell recovery are urgently needed. Magnetic iron oxide nanoparticles show promising potential for various biomedical applications because of their advantages such as high biocompatibility and cost-effectiveness. Herein, a new facile platform for macromolecular delivery was developed based on the photothermal properties of porous magnetic iron oxide nanoparticles (P-MNPs). The near-infrared radiation (NIR) absorption behavior of P-MNPs remarkably facilitates the delivery of macromolecules into cells while maintaining high cell viability. Furthermore, the assistance of polycationic polyethylenimine improves the efficiency of DNA delivery. Most importantly, the cells could be easily recovered after macromolecular delivery by trypsinization, which is of great significance for further practical application of the delivery system. The facile and cost-effective platform proposed in this work provides a new avenue for the utilization of P-MNPs in macromolecular delivery.

中文翻译:

使用多孔磁性氧化铁纳米材料作为用于大分子递送的便捷光穿孔纳米平台

对于生物医学应用而言,外源大分子例如功能蛋白,抗体,多糖和核酸向活细胞内的细胞内递送是令人关注的。即使为此付出了巨大的努力,仍迫切需要能够提供出色的细胞内递送性能并易于细胞恢复的通用递送系统。磁性氧化铁纳米颗粒由于具有诸如高生物相容性和成本效益之类的优势,因此在各种生物医学应用中显示出令人鼓舞的潜力。本文中,基于多孔磁性氧化铁纳米粒子(P-MNPs)的光热性能,开发了一种新的用于大分子递送的简便平台。P-MNP的近红外辐射(NIR)吸收行为显着促进了大分子向细胞内的递送,同时保持了高细胞活力。此外,聚阳离子聚乙烯亚胺的辅助提高了DNA递送的效率。最重要的是,在大分子递送后通过胰蛋白酶消化可以容易地回收细胞,这对于递送系统的进一步实际应用具有重要意义。在这项工作中提议的便捷且具有成本效益的平台为在大分子递送中利用P-MNP提供了新途径。这对于输送系统的进一步实际应用具有重要意义。在这项工作中提议的便捷且具有成本效益的平台为在大分子递送中利用P-MNP提供了新途径。这对于输送系统的进一步实际应用具有重要意义。在这项工作中提议的便捷且具有成本效益的平台为在大分子递送中利用P-MNP提供了新途径。
更新日期:2018-06-14
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