An aerolysin nanopore interface was introduced as a molecular machine to electrically read out the real-time photo-controlled motion of an azobenzene-geared DNA train with high spatial and temporal resolution. Under alternating UV and visible irradiation, each DNA train performed two regulated speeds of 1.9 and 6.3 bases/s corresponding to trans and cis states, respectively, with readily identified current signals. Each train type fell into the ultra-narrow current population with a full-width half maximum of 0.2–0.4 pA. The combination of a model molecular machine system and powerful aerolysin interface enabled the motions of every artificial molecular machine to be followed in real time.

引入了溶菌素纳米孔界面作为分子机器，以电读出具有高时空分辨率的偶氮苯齿轮DNA序列的实时光控运动。下交替UV和可见光照射下，每一个DNA列车执行的1.9和6.3个碱基两个被调节的速度/ s的对应于反式和CIS状态，分别与容易地识别当前信号。每种类型的火车都属于超窄带当前人群，其半峰全宽为0.2-0.4 pA。模型分子机器系统与强大的溶血素界面的结合使每台人造分子机器的运动得以实时跟踪。