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Methods and Technologies for Exosome Isolation and Characterization
Small Methods ( IF 12.4 ) Pub Date : 2018-06-12 , DOI: 10.1002/smtd.201800021
Mengdi Zhang 1 , Ke Jin 1 , Liang Gao 2 , Zhengkui Zhang 1 , Fang Li 1 , Fangfang Zhou 2 , Long Zhang 1
Affiliation  

Exosomes, a specific subclass of the extracellular vesicles secreted by most cell types, play an important role in cell–cell communication by transporting diverse content including protein, mRNA, miRNA, and DNA. This cargo is closely associated with the pathogenesis of most human malignancies. Therefore, it is becoming an urgent demand to be able to isolate exosomes in a simple, efficient, and economical way for scientific research and clinical diagnosis. Here, several conventional and novel nano‐based techniques of exosome isolation and characterization are summarized, and the advantages and disadvantages among them are compared, with the hope that researchers will be provided with an overview in this field to detect and isolate exosomes in a suitable manner, matching the subsequent experiments.

中文翻译:

外泌体分离和表征的方法和技术

外来体是大多数细胞类型分泌的细胞外囊泡的特定亚类,它通过转运各种内容(包括蛋白质,mRNA,miRNA和DNA)在细胞间通信中发挥重要作用。这种货物与大多数人类恶性肿瘤的发病机理密切相关。因此,迫切需要能够以简单,有效和经济的方式分离外泌体用于科学研究和临床诊断。在此,总结了几种常规的和新颖的基于纳米的外泌体分离和表征技术,并比较了它们之间的优缺点,希望为研究人员提供该领域的概述,以检测和分离合适的外泌体。方式,匹配后续实验。
更新日期:2018-06-12
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